Mechanisms of action of cholecystokinin in the canine gastrointestinal tract: Role of vasoactive intestinal peptide and nitric oxide

P. Vergara, Z. Woskowska, S. Cipris, J. E.T. Fox-Threlkeld, E. E. Daniel

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    20 Citations (Scopus)

    Abstract

    This study defined the cholecystokinin (CCK)/gastrin receptor subtypes at which CCK octapeptide (CCK(B)) and gastrin 17 (G17) act on motor functions of the intact canine gastrointestinal tract. In the antrum, studies of tachyphylaxis and effects of antagonists showed that i.a. G17 acted through CCK(B) receptors to activate contractions, whereas CCK(B) acted through A and B receptor subtypes to produce contractions. In the duodenum, i.a. G17 caused dose-dependent inhibition of electrical fieldstimulated contractions, apparently by release of nitric oxide [blocked by N-nitro-L- arginine (L-NNA) or NG-L-arginine methyl ester]. These inhibitory effects were abolished by YMO22 (a CCK(B) antagonist) but not by L-364,718 (a CCKA antagonist). However, i.a. CCK(B) increased electrical field-stimulated contractions and L-364,718 reversed this effect. In isolated perfused segments of distal intestine, CCK(B) caused inhibition and excitation and released vasoactive intestinal peptide (VIP) into the venous effluent. CCK tetrapeptide and G17 had inconsistent effects. Excitation and VIP release were inhibited by L-364,718. L-NNA potentiated excitatory responses and abolished inhibitory responses. Tetrodotoxin and atropine abolished and hexamethonium reduced excitatory responses to CCK(B), but L-NNA restored contractions after atropine treatment. Hexamethonium or L-NNA (but not atropine) reduced VIP release; CCK(B) still enhanced it. L-364,718 abolished hexamethonium-resistant contractions and VIP release. Thus, CCK/gastrin peptides act on neural receptors in intact canine gastrointestinal tract. In antrum, activation of neural CCK(A) or CCK(B) receptors initiates contractions. In intestine, CCK(A) receptors at pre- and postjunctional sites in enteric nerves mediate acetylcholine and VIP release. CCK(B) receptors mediate release of an inhibitory mediator, apparently nitric oxide, from postjunctional sites.
    Original languageEnglish
    Pages (from-to)306-316
    JournalJournal of Pharmacology and Experimental Therapeutics
    Volume279
    Issue number1
    Publication statusPublished - 1 Oct 1996

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