Mechanisms mediating the effects of cholecystokinin on avian small intestine longitudinal smooth muscle

M. T. Martín; E. Fernández; A. G. Fernández; E. Goñalons

doi:10.1016/0167-0115(94)90198-8

Mechanisms mediating the effects of cholecystokinin on avian small intestine longitudinal smooth muscle

M. T. Martín, E. Fernández, A. G. Fernández, E. Goñalons

Research output: Contribution to journal › Article › Research › peer-review

10 Citations (Scopus)

Abstract

The aims of this study were (1) to define the effects of CCK-8s and related peptides on chicken ileum longitudinal smooth muscle and (2) to explore the mechanisms by which such effects occur. The effects of CCK-8s were assayed in vitro on chicken longitudinal ileal strips. CCK-8s produced contraction of ileal strips (EC50 8.8·10-9 M). CCK-8ns and CCK-4 did not have remarkable contractile effects even when added at concentrations 200-times higher than the EC50 for CCK-8s. L365,260 slightly inhibited the effects of CCK-8s whereas L364,718 was ineffective. Tetrodotoxin (10-6 M) markedly decreased the effects of CCK-8s. Atropine (10-6 M) did not modify the neurally mediated effects of CCK-8s, whereas ketanserin (10-5 M) decreased the response to CCK-8s. Substance P-desensitized preparations exhibited reduced responses to CCK-8s. Our results indicate that CCK receptors present in chicken ileum behave similarly but not identically to the CCK-A receptor described in mammals. Most of these CCK receptors are neurally located but a minor proportion is also present on smooth muscle. The neurally mediated response to CCK-8s does not involve cholinergic mechanisms, but serotonin and substance P releasing neurons. © 1994.

Original language	English
Pages (from-to)	91-99
Journal	Regulatory Peptides
Volume	51
Issue number	2
DOIs	https://doi.org/10.1016/0167-0115(94)90198-8
Publication status	Published - 5 May 1994

Keywords

Bird
Chicken
Gastrointestinal motility
L364,718
L365,260
Substance P

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title = "Mechanisms mediating the effects of cholecystokinin on avian small intestine longitudinal smooth muscle",

abstract = "The aims of this study were (1) to define the effects of CCK-8s and related peptides on chicken ileum longitudinal smooth muscle and (2) to explore the mechanisms by which such effects occur. The effects of CCK-8s were assayed in vitro on chicken longitudinal ileal strips. CCK-8s produced contraction of ileal strips (EC50 8.8·10-9 M). CCK-8ns and CCK-4 did not have remarkable contractile effects even when added at concentrations 200-times higher than the EC50 for CCK-8s. L365,260 slightly inhibited the effects of CCK-8s whereas L364,718 was ineffective. Tetrodotoxin (10-6 M) markedly decreased the effects of CCK-8s. Atropine (10-6 M) did not modify the neurally mediated effects of CCK-8s, whereas ketanserin (10-5 M) decreased the response to CCK-8s. Substance P-desensitized preparations exhibited reduced responses to CCK-8s. Our results indicate that CCK receptors present in chicken ileum behave similarly but not identically to the CCK-A receptor described in mammals. Most of these CCK receptors are neurally located but a minor proportion is also present on smooth muscle. The neurally mediated response to CCK-8s does not involve cholinergic mechanisms, but serotonin and substance P releasing neurons. {\textcopyright} 1994.",

keywords = "Bird, Chicken, Gastrointestinal motility, L364,718, L365,260, Substance P",

author = "Mart{\'i}n, {M. T.} and E. Fern{\'a}ndez and Fern{\'a}ndez, {A. G.} and E. Go{\~n}alons",

year = "1994",

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AU - Martín, M. T.

AU - Fernández, E.

AU - Fernández, A. G.

AU - Goñalons, E.

PY - 1994/5/5

Y1 - 1994/5/5

N2 - The aims of this study were (1) to define the effects of CCK-8s and related peptides on chicken ileum longitudinal smooth muscle and (2) to explore the mechanisms by which such effects occur. The effects of CCK-8s were assayed in vitro on chicken longitudinal ileal strips. CCK-8s produced contraction of ileal strips (EC50 8.8·10-9 M). CCK-8ns and CCK-4 did not have remarkable contractile effects even when added at concentrations 200-times higher than the EC50 for CCK-8s. L365,260 slightly inhibited the effects of CCK-8s whereas L364,718 was ineffective. Tetrodotoxin (10-6 M) markedly decreased the effects of CCK-8s. Atropine (10-6 M) did not modify the neurally mediated effects of CCK-8s, whereas ketanserin (10-5 M) decreased the response to CCK-8s. Substance P-desensitized preparations exhibited reduced responses to CCK-8s. Our results indicate that CCK receptors present in chicken ileum behave similarly but not identically to the CCK-A receptor described in mammals. Most of these CCK receptors are neurally located but a minor proportion is also present on smooth muscle. The neurally mediated response to CCK-8s does not involve cholinergic mechanisms, but serotonin and substance P releasing neurons. © 1994.

AB - The aims of this study were (1) to define the effects of CCK-8s and related peptides on chicken ileum longitudinal smooth muscle and (2) to explore the mechanisms by which such effects occur. The effects of CCK-8s were assayed in vitro on chicken longitudinal ileal strips. CCK-8s produced contraction of ileal strips (EC50 8.8·10-9 M). CCK-8ns and CCK-4 did not have remarkable contractile effects even when added at concentrations 200-times higher than the EC50 for CCK-8s. L365,260 slightly inhibited the effects of CCK-8s whereas L364,718 was ineffective. Tetrodotoxin (10-6 M) markedly decreased the effects of CCK-8s. Atropine (10-6 M) did not modify the neurally mediated effects of CCK-8s, whereas ketanserin (10-5 M) decreased the response to CCK-8s. Substance P-desensitized preparations exhibited reduced responses to CCK-8s. Our results indicate that CCK receptors present in chicken ileum behave similarly but not identically to the CCK-A receptor described in mammals. Most of these CCK receptors are neurally located but a minor proportion is also present on smooth muscle. The neurally mediated response to CCK-8s does not involve cholinergic mechanisms, but serotonin and substance P releasing neurons. © 1994.

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KW - Chicken

KW - Gastrointestinal motility

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