© 2018 The Author(s). Background: Urea and creatinine in saliva have been reported to be possible markers of chronic kidney disease (CKD) in humans. The aim of this study was to assess if urea and creatinine could be measured in canine saliva, and to evaluate their possible changes in situations of CKD. Results: The spectrophotometric assays for urea and creatinine measurements in saliva of dogs showed intra- and inter-assay imprecision lower than 12% and coefficients of correlation close to 1 in linearity under dilution tests. Healthy dogs showed median salivary concentrations of urea of 39.6mg/dL and creatinine of 0.30mg/dL, whereas dogs with CKDshowed median salivary urea of 270.1mg/dL and creatinine of 1.86mg/dL. Positive high correlations were found between saliva and serum activities of the two analytes (urea, r=0.909; P<0.001; creatinine, r=0.819; P<0.001). Conclusions: Urea and creatinine concentrations can be measured in canine saliva with commercially available spectrophotometric assays. Bothanalytes showed higher values in saliva of dogs with CKD compared with healthy dogs and their values were highly correlated with those in serumin our study conditions.