Measurement of p-nitrophenyl acetate esterase activity (EA), total antioxidant capacity (TAC), total oxidant status (TOS) and acetylcholinesterase (AChE) in gills and digestive gland of Mytilus galloprovincialis exposed to binary mixtures of Pb, Cd and Cu

Lorena Franco-Martinez, Diego Romero, José A. García-Navarro, Fernando Tecles, Mariana Teles, Asta Tvarijonaviciute

Research output: Contribution to journalArticleResearchpeer-review

14 Citations (Scopus)

Abstract

© 2016, Springer-Verlag Berlin Heidelberg. The aims of the present work were (1) to evaluate oxidative stress biomarkers and AChE in two tissues of wild mussel (Mytilus galloprovincialis) of high biochemical activity and accumulation capacity (gills and digestive gland) and (2) to study the behaviour of these biomarkers in presence of heavy metals. For this, EA, TOS, TAC and AChE were measured in tissues of mussels exposed to binary combination of Pb, Cd and Cu. Mussels (n = 36) were exposed to one of the binary mixtures of Pb (1000 μg L −1 ), Cd (100 μg L −1 ) and Cu (100 μg L −1 ) for 7 days, under controlled conditions. Gills and digestive gland were extracted and frozen at −80 °C until analysis. The automatic methods employed for the measurement of EA, TAC, TOS and AChE in M. galloprovincialis revealed higher levels of these biomarkers in digestive gland than gills. Study results suggest that gills would be the tissue of election for study oxidative stress markers, whereas digestive tissue should be selected for AChE measurements in case of evaluation of combined metal toxicity in mussels.
Original languageEnglish
Pages (from-to)25385-25392
JournalEnvironmental Science and Pollution Research
Volume23
Issue number24
DOIs
Publication statusPublished - 1 Dec 2016

Keywords

  • Acetylcholinesterase
  • Cadmium
  • Copper
  • Lead
  • Oxidative stress
  • Wild mussels

Fingerprint Dive into the research topics of 'Measurement of p-nitrophenyl acetate esterase activity (EA), total antioxidant capacity (TAC), total oxidant status (TOS) and acetylcholinesterase (AChE) in gills and digestive gland of Mytilus galloprovincialis exposed to binary mixtures of Pb, Cd and Cu'. Together they form a unique fingerprint.

Cite this