Measurement of intracellular pH of maize seeds (Zea mays) during germination by 31P nuclear magnetic resonance spectroscopy.

I. Barba, C. Gasparovic, M. E. Cabañas, J. Alonso, I. Murillo, B. San Segundo, C. Arús

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13 Citations (Scopus)

Abstract

The 31P NMR spectra of germinating maize seeds showed a single broad resonance that shifted its position as germination proceeded (studied between 0 and 10 days). The resonance was shown to originate from the phosphate groups of phytine (Mg2+, Ca2+ and K+ salt of myoinositol hexakisphosphate) in a subcellular compartment of the embryo scutellar cells. A series of calibration curves for the chemical shift dependence of the phytate resonance in the presence of Mg2+ and Ca2+ were constructed. These calibration curves allowed us to determine that an acidification of the phytate containing compartment in the seed embryo takes place, reaching a minimum at about pH 4 after three days of germination. This acidification could be important in allowing phytate solubilization for export to growing parts of the maize seedling.
Original languageEnglish
Pages (from-to)609-620
JournalCellular and molecular biology (Noisy-le-Grand, France)
Volume43
Issue number5
Publication statusPublished - 1 Jan 1997

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