Measurement of cell volume of phototrophic bacteria in pure cultures and natural samples: phase contrast, epifluorescence and particle sizing

Núria Gaju, Ricardo Guerrero, Carlos Pedrós-Alió

Research output: Contribution to journalArticleResearchpeer-review

6 Citations (Scopus)

Abstract

Cell volumes of different purple phototrophic bacteria were measured using several techniques: Coulter counter, phase contrast and epifluorescence microscopy. Volumes of Chromatium warmingii, C. minutissimum, Thiocapsa roseopersicina, and Thiocystis gelatinosa were measured as the organisms were accumulating sulfur. Cell volumes of Rhodobacter capsulatus were measured under different growth conditions including both anaerobically in the light and aerobically in the dark. Size distributions were flatter and more irregular by phase contrast microscopy than by Coulter counter. This latter technique could not be used in many cases, however, because phototrophic bacteria associate to form chains and aggregates of cells. In addition, Coulter counter measurements for organisms with capsules gave volumes intermediate between the volume of the cell and the volume of the capsule, as measured by phase contrast microscopy. Epifluorescence gave similar results to phase contrast if organic solvents were not used in the preparation of samples. Finally, cell volume of two phototrophic bacteria was shown to change both with depth and with season in a natural system. © 1989.
Original languageEnglish
Pages (from-to)295-302
JournalFEMS Microbiology Letters
Volume62
Issue number5
DOIs
Publication statusPublished - 1 Jan 1989

Keywords

  • Chromatium
  • Growth medium
  • Inclusions
  • Light intensity
  • Rhodobacter
  • Thiocapsa
  • Thiocystis

Fingerprint Dive into the research topics of 'Measurement of cell volume of phototrophic bacteria in pure cultures and natural samples: phase contrast, epifluorescence and particle sizing'. Together they form a unique fingerprint.

Cite this