Mammalian orthologous PCR of the putative TM4 of the NRAMP1 gene and molecular characterisation of this region in dogs

Laura Altet, O. Francino, A. Sánchez

    Research output: Contribution to journalArticleResearchpeer-review

    1 Citation (Scopus)

    Abstract

    Orthologous gene-specific PCR primers have been designed to amplify the putative transmembrane domain (TM) 4 of the Nramp1 gene in mammals. Sequencing of the dog PCR fragment showed to be Nramp1 gene and it was used as a probe for Southern blot analysis, confirming the specific amplification of this gene also in other species. In order to analyse the relationship between Nramp and Leishmaniosis in dogs, a PCR-RFLP protocol has been optimised to look for TM4 polymorphism. Results showed a conserved Gly169residue in all animals tested and only two intronic polymorphisms.
    Original languageEnglish
    Pages (from-to)126-129
    JournalArchiv fur Tierzucht
    Volume42
    Publication statusPublished - 1 Dec 1999

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