Mammalian orthologous PCR of the putative TM4 of the NRAMP1 gene and molecular characterisation of this region in dogs

Laura Altet; O. Francino; A. Sánchez

Mammalian orthologous PCR of the putative TM4 of the NRAMP1 gene and molecular characterisation of this region in dogs

Laura Altet, O. Francino, A. Sánchez

Research output: Contribution to journal › Article › Research › peer-review

Abstract

Orthologous gene-specific PCR primers have been designed to amplify the putative transmembrane domain (TM) 4 of the Nramp1 gene in mammals. Sequencing of the dog PCR fragment showed to be Nramp1 gene and it was used as a probe for Southern blot analysis, confirming the specific amplification of this gene also in other species. In order to analyse the relationship between Nramp and Leishmaniosis in dogs, a PCR-RFLP protocol has been optimised to look for TM4 polymorphism. Results showed a conserved Gly169residue in all animals tested and only two intronic polymorphisms.

Original language	English
Pages (from-to)	126-129
Journal	Archiv fur Tierzucht
Volume	42
Publication status	Published - 1 Dec 1999

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title = "Mammalian orthologous PCR of the putative TM4 of the NRAMP1 gene and molecular characterisation of this region in dogs",

abstract = "Orthologous gene-specific PCR primers have been designed to amplify the putative transmembrane domain (TM) 4 of the Nramp1 gene in mammals. Sequencing of the dog PCR fragment showed to be Nramp1 gene and it was used as a probe for Southern blot analysis, confirming the specific amplification of this gene also in other species. In order to analyse the relationship between Nramp and Leishmaniosis in dogs, a PCR-RFLP protocol has been optimised to look for TM4 polymorphism. Results showed a conserved Gly169residue in all animals tested and only two intronic polymorphisms.",

author = "Laura Altet and O. Francino and A. S{\'a}nchez",

year = "1999",

month = dec,

day = "1",

language = "English",

volume = "42",

pages = "126--129",

journal = "Archives Animal Breeding",

issn = "0003-9438",

publisher = "Forschungsinstitut fuer die Biologie Landwirtschaftlicher Nutztiere",

}

TY - JOUR

T1 - Mammalian orthologous PCR of the putative TM4 of the NRAMP1 gene and molecular characterisation of this region in dogs

AU - Altet, Laura

AU - Francino, O.

AU - Sánchez, A.

PY - 1999/12/1

Y1 - 1999/12/1

N2 - Orthologous gene-specific PCR primers have been designed to amplify the putative transmembrane domain (TM) 4 of the Nramp1 gene in mammals. Sequencing of the dog PCR fragment showed to be Nramp1 gene and it was used as a probe for Southern blot analysis, confirming the specific amplification of this gene also in other species. In order to analyse the relationship between Nramp and Leishmaniosis in dogs, a PCR-RFLP protocol has been optimised to look for TM4 polymorphism. Results showed a conserved Gly169residue in all animals tested and only two intronic polymorphisms.

AB - Orthologous gene-specific PCR primers have been designed to amplify the putative transmembrane domain (TM) 4 of the Nramp1 gene in mammals. Sequencing of the dog PCR fragment showed to be Nramp1 gene and it was used as a probe for Southern blot analysis, confirming the specific amplification of this gene also in other species. In order to analyse the relationship between Nramp and Leishmaniosis in dogs, a PCR-RFLP protocol has been optimised to look for TM4 polymorphism. Results showed a conserved Gly169residue in all animals tested and only two intronic polymorphisms.

M3 - Article

VL - 42

SP - 126

EP - 129

JO - Archives Animal Breeding

JF - Archives Animal Breeding

SN - 0003-9438

ER -