Mammalian metallopeptidase inhibition at the defense barrier of Ascaris parasite

Laura Sanglas, Francesc X. Aviles, Robert Huber, F. Xavier Gomis-Rüthe, Joan L. Arolas

Research output: Contribution to journalArticleResearchpeer-review

35 Citations (Scopus)

Abstract

Roundworms of the genus Ascaris are common parasites of the human gastrointestinal tract. A battery of selective inhibitors protects them from host enzymes and the immune system. Here, a metallocarboxypeptidase (MCP) inhibitor, ACI, was identified in protein extracts from Ascaris by intensity-fading MALDI-TOF mass spectrometry. The 67-residue amino acid sequence of ACI showed no significant homology with any known protein. Heterologous overexpression and purification of ACI rendered a functional molecule with nanomolar equilibrium dissociation constants against MCPs, which denoted a preference for digestive and mast cell A/B-type MCPs. Western blotting and immunohistochemistry located ACI in the body wall, intestine, female reproductive tract, and fertilized eggs of Ascaris, in accordance with its target specificity. The crystal structure of the complex of ACI with human carboxypeptidase A1, one of its potential targets in vivo, revealed a protein with a fold consisting of two tandem homologous domains, each containing a β-ribbon and two disulfide bonds. These domains are connected by an α-helical segment and a fifth disulfide bond. Binding and inhibition are exerted by the C-terminal tail, which enters the funnel-like active-site cavity of the enzyme and approaches the catalytic zinc ion. The findings reported provide a basis for the biological function of ACI, which may be essential for parasitic survival during infection. © 2009 by The National Academy of Sciences of the USA.
Original languageEnglish
Pages (from-to)1743-1747
JournalProceedings of the National Academy of Sciences of the United States of America
Volume106
Issue number6
DOIs
Publication statusPublished - 10 Feb 2009

Keywords

  • Ascariasis
  • Crystal structure
  • Host resistance
  • Immunolocalization
  • Metallocarboxypeptidase inhibitor

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