Mammalian class IV alcohol dehydrogenase (stomach alcohol dehydrogenase): Structure, origin, and correlation with enzymology

Xavier Parés, Ella Cederlund, Alberto Moreno, Lars Hjelmqvist, Jaume Farrés, Hans Jörnvall

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45 Citations (Scopus)

Abstract

The structure of a mammalian class IV alcohol dehydrogenase has been determined by peptide analysis of the protein isolated from rat stomach. The structure indicates that the enzyme constitutes a separate alcohol dehydrogenase class, in agreement with the distinct enzymatic properties; the class IV enzyme is somewhat closer to class I (the 'classical' liver alcohol dehydrogenase; ≃68% residue identities) than to the other classes (II, III, and V; ≃60% residue identities), suggesting that class IV might have originated through duplication of an early vertebrate class I gene. The activity of the class IV protein toward ethanol is even higher than that of the classical liver enzyme. Both K(m) and k(cat) values are high, the latter being the highest of any class characterized so far. Structurally, these properties are correlated with replacements at the active site, affecting both substrate and coenzyme binding. In particular, Ala-294 (instead of valine) results in increased space in the middle section of the substrate cleft, Gly-47 (instead of a basic residue) results in decreased charge interactions with the coenzyme pyrophosphate, and Tyr-363 (instead of a basic residue) may also affect coenzyme binding. In combination, these exchanges are compatible with a promotion of the off dissociation and an increased turnover rate. In contrast, residues at the inner part of the substrate cleft are bulky, accounting for low activity toward secondary alcohols and cyclohexanol. Exchanges at positions 259-261 involve minor shifts in glycine residues at a reverse turn in the coenzyme-binding fold. Clearly, class IV is distinct in structure, ethanol turnover, stomach expression, and possible emergence from class I.
Original languageEnglish
Pages (from-to)1893-1897
JournalProceedings of the National Academy of Sciences of the United States of America
Volume91
Issue number5
DOIs
Publication statusPublished - 1 Mar 1994

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