Magnetically separable polymer (Mag-MIP) for selective analysis of biotin in food samples

Rosario Josefina Uzuriaga-Sánchez, Sabir Khan, Ademar Wong, Gino Picasso, Maria Isabel Pividori, Maria Del Pilar Taboada Sotomayor

Research output: Contribution to journalArticleResearchpeer-review

48 Citations (Scopus)

Abstract

© 2015 Elsevier Ltd. All rights reserved. This work presents an efficient method for the preparation of magnetic nanoparticles modified with molecularly imprinted polymers (Mag-MIP) through core-shell method for the determination of biotin in milk food samples. The functional monomer acrylic acid was selected from molecular modeling, EGDMA was used as cross-linking monomer and AIBN as radical initiator. The Mag-MIP and Mag-NIP were characterized by FTIR, magnetic hysteresis, XRD, SEM and N<inf>2</inf>-sorption measurements. The capacity of Mag-MIP for biotin adsorption, its kinetics and selectivity were studied in detail. The adsorption data was well described by Freundlich isotherm model with adsorption equilibrium constant (K<inf>F</inf>) of 1.46 mL g<sup>-1</sup>. The selectivity experiments revealed that prepared Mag-MIP had higher selectivity toward biotin compared to other molecules with different chemical structure. The material was successfully applied for the determination of biotin in diverse milk samples using HPLC for quantification of the analyte, obtaining the mean value of 87.4% recovery.
Original languageEnglish
Pages (from-to)460-467
JournalFood Chemistry
Volume190
DOIs
Publication statusPublished - 10 Jun 2016

Keywords

  • Biotin
  • Imprinted polymers
  • Magnetic nanoparticles
  • Magnetic-MIP
  • Milk

Fingerprint Dive into the research topics of 'Magnetically separable polymer (Mag-MIP) for selective analysis of biotin in food samples'. Together they form a unique fingerprint.

  • Cite this