Abstract
© 2016 Elsevier B.V. Magnetic separation based on biologically-modified magnetic particles is a preconcentration procedure commonly integrated in magneto actuated platforms for the detection of a huge range of targets. However, the main drawback of this material is the low stability and high cost. In this work, a novel hybrid molecularly-imprinted polymer with magnetic properties is presented with affinity towards biotin and biotinylated biomolecules. During the synthesis of the magneto core-shell particles, biotin was used as a template. The characterization of this material by microscopy techniques including SEM, TEM and confocal microscopy is presented. The application of the magnetic-MIPs for the detection of biotin and biotinylated DNA in magneto-actuated platforms is also described for the first time. The magnetic-MIP showed a significant immobilization capacity of biotinylated molecules, giving rise to a cheaper and a robust method (it is not required to be stored at 4 °C) with high binding capacity for the separation and purification under magnetic actuation of a wide range of biotinylated molecules, and their downstream application including determination of their specific targets.
Original language | English |
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Pages (from-to) | 101-108 |
Journal | Biosensors and Bioelectronics |
Volume | 88 |
DOIs | |
Publication status | Published - 15 Feb 2017 |
Keywords
- biotinylated biomolecules
- immunomagnetic separation
- magnetic molecularly-imprinted polymer
- magnetic particles
- magneto-actuated immunoassay