Magnetic molecularly imprinted polymer for the isolation and detection of biotin and biotinylated biomolecules

A. Ben Aissa, A. Herrera-Chacon, R. R. Pupin, M. D.P.T. Sotomayor, M. I. Pividori

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43 Citations (Scopus)


© 2016 Elsevier B.V. Magnetic separation based on biologically-modified magnetic particles is a preconcentration procedure commonly integrated in magneto actuated platforms for the detection of a huge range of targets. However, the main drawback of this material is the low stability and high cost. In this work, a novel hybrid molecularly-imprinted polymer with magnetic properties is presented with affinity towards biotin and biotinylated biomolecules. During the synthesis of the magneto core-shell particles, biotin was used as a template. The characterization of this material by microscopy techniques including SEM, TEM and confocal microscopy is presented. The application of the magnetic-MIPs for the detection of biotin and biotinylated DNA in magneto-actuated platforms is also described for the first time. The magnetic-MIP showed a significant immobilization capacity of biotinylated molecules, giving rise to a cheaper and a robust method (it is not required to be stored at 4 °C) with high binding capacity for the separation and purification under magnetic actuation of a wide range of biotinylated molecules, and their downstream application including determination of their specific targets.
Original languageEnglish
Pages (from-to)101-108
JournalBiosensors and Bioelectronics
Publication statusPublished - 15 Feb 2017


  • biotinylated biomolecules
  • immunomagnetic separation
  • magnetic molecularly-imprinted polymer
  • magnetic particles
  • magneto-actuated immunoassay


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