Abstract
© Springer-Verlag 2014. While magnetic bead (MB)-based bioassays have been implemented in integrated devices, their handling on-chip is normally either not optimal - i.e. only trapping is achieved, with aggregation of the beads - or requires complex actuator systems. Herein, we describe a simple and low-cost magnetic actuator to trap and move MBs within a microfluidic chamber in order to enhance the mixing of a MB-based reaction. The magnetic actuator consists of a CD-shaped plastic unit with an arrangement of embedded magnets which, when rotating, generate the mixing. Themagnetic actuator has been used to enhance the amplification reaction of an enzyme-linked fluorescence immunoassay to detect Escherichia coli O157:H7 whole cells, an enterohemorrhagic strain, which have caused several outbreaks in food andwater samples. A 2.7-fold sensitivity enhancement was attained with a detection limit of 603 colony-forming units (CFU)/mL, when employing the magnetic actuator.
Original language | English |
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Pages (from-to) | 6607-6616 |
Journal | Analytical and Bioanalytical Chemistry |
Volume | 406 |
Issue number | 26 |
DOIs | |
Publication status | Published - 2 Oct 2014 |
Keywords
- Immunoassay
- Lab on a chip
- Magnetic actuator
- Magnetic beads
- Magnetic mixing