TY - JOUR
T1 - Lysyl oxidase-like 2 (LOXL2) oxidizes trimethylated lysine 4 in histone H3
AU - Herranz, Nicolás
AU - Dave, Natàlia
AU - Millanes-Romero, Alba
AU - Pascual-Reguant, Laura
AU - Morey, Lluis
AU - Díaz, Víctor M.
AU - Lórenz-Fonfría, Víctor
AU - Gutierrez-Gallego, Ricardo
AU - Jerónimo, Celia
AU - Iturbide, Ane
AU - Di Croce, Luciano
AU - García de Herreros, Antonio
AU - Peiró, Sandra
PY - 2016/12/1
Y1 - 2016/12/1
N2 - © 2016 Federation of European Biochemical Societies Methylation of histone H3 lysine 4 is linked to active transcription and can be removed by LSD1 or the JmjC domain-containing proteins by amino-oxidation or hydroxylation, respectively. Here we describe that its deamination can be catalyzed by lysyl oxidase-like 2 protein (LOXL2), presenting an unconventional chemical mechanism for H3K4 modification. Infrared spectroscopy and mass spectrometry analyses demonstrated that recombinant LOXL2 specifically deaminates trimethylated H3K4. Moreover, by regulating H3K4me3 deamination, LOXL2 activity is linked with the transcriptional control of the CDH1 gene. These results reveal the existence of further H3 modification as well as a novel mechanism for H3K4me3 demethylation. Database: The GEO accession number for the data referred to this paper is GSE35600.
AB - © 2016 Federation of European Biochemical Societies Methylation of histone H3 lysine 4 is linked to active transcription and can be removed by LSD1 or the JmjC domain-containing proteins by amino-oxidation or hydroxylation, respectively. Here we describe that its deamination can be catalyzed by lysyl oxidase-like 2 protein (LOXL2), presenting an unconventional chemical mechanism for H3K4 modification. Infrared spectroscopy and mass spectrometry analyses demonstrated that recombinant LOXL2 specifically deaminates trimethylated H3K4. Moreover, by regulating H3K4me3 deamination, LOXL2 activity is linked with the transcriptional control of the CDH1 gene. These results reveal the existence of further H3 modification as well as a novel mechanism for H3K4me3 demethylation. Database: The GEO accession number for the data referred to this paper is GSE35600.
KW - epigenetics
KW - histone modification
KW - lysyl oxidase-like 2
KW - snail1
KW - transcriptional regulation
U2 - 10.1111/febs.13922
DO - 10.1111/febs.13922
M3 - Article
VL - 283
SP - 4263
EP - 4273
IS - 23
ER -