Low persistence of radiation-induced centromere positive and negative micronuclei in cultured human cells

M. J. Ramírez, J. Surrallés, S. Puerto, A. Creus, R. Marcos

Research output: Contribution to journalArticleResearchpeer-review

20 Citations (Scopus)

Abstract

The micronucleus (MN) assay is widely used both in genetic toxicology and in the biomonitoring of human populations. Lymphocytes, cell lines, and bone marrow and epithelial cells are usually employed as target systems in such studies. However, little effort has been done to assess the persistence of MN in highly proliferative cells. To study the behaviour of MN containing whole chromosomes or acentric fragments, we have performed a time course experiment on the persistence of γ-ray (3 Gy) induced MN in a human lymphoblastoid cell line. The frequency and content of MN were analyzed 1, 3, 7, 14, and 56 days after irradiation by pancentromeric fluorescence in situ hybridization (FISH). We observed a clear induction of both centromere positive and negative MN at completion of the first mitotic division. The frequency of both types of MN drastically declined to basal levels 7 days after irradiation with an identical kinetics. We therefore conclude that centromere positive and negative MN are highly unstable upon cell division, indicating that the MN assay could not be a good biomarker of DNA damage induced by acute treatments in highly proliferative cells. The implication of our findings in biomonitoring and in genotoxicity studies is discussed. Copyright (C) 1999 Elsevier Science B.V.
Original languageEnglish
Pages (from-to)163-169
JournalMutation Research - Genetic Toxicology and Environmental Mutagenesis
Volume440
Issue number2
DOIs
Publication statusPublished - 6 Apr 1999

Keywords

  • Centromere
  • Fluorescence in situ hybridization (FISH)
  • Micronucleus
  • Persistence
  • Radiation-induced

Fingerprint Dive into the research topics of 'Low persistence of radiation-induced centromere positive and negative micronuclei in cultured human cells'. Together they form a unique fingerprint.

  • Cite this