Low E-prostanoid 2 receptor levels and deficient induction of the IL-1β/IL-1 type i receptor/COX-2 pathway: Vicious circle in patients with aspirin-exacerbated respiratory disease

Liliana Machado-Carvalho, Margarita Martín, Rosa Torres, Marta Gabasa, Isam Alobid, Joaquim Mullol, Laura Pujols, Jordi Roca-Ferrer, Cesar Picado

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    28 Citations (Scopus)

    Abstract

    © 2015 American Academy of Allergy, Asthma & Immunology. Background We hypothesized that the 2 reported alterations in aspirin-exacerbated respiratory disease (AERD), reduced expression/production of COX-2/prostaglandin (PG) E2 and diminished expression of E-prostanoid (EP) 2 receptor, are closely linked. Objective We sought to determine the mechanisms involved in the altered regulation of the COX pathway in patients with AERD. Methods Fibroblasts were obtained from nasal mucosa; samples of control subjects (NM-C, n = 8) and from nasal polyps from patients with aspirin-exacerbated respiratory disease (NP-AERD, n = 8). Expression of the autocrine loop components regulating PGE2 production and signaling, namely IL-1 type I receptor (IL-1RI), COX-2, microsomal prostaglandin E synthase 1 (mPGES-1), and EP receptors, was assessed at baseline and after stimulation with IL-1β, PGE2, and specific EP receptor agonists. Results Compared with NM-C fibroblasts, basal expression levels of IL-1RI and EP2 receptor were lower in NP-AERD fibroblasts. IL-1β-induced IL-1RI, COX-2, and mPGES-1 expression levels were also lower in these cells. Levels of IL-1RI positively correlated with COX-2 and mPGES-1 expression in both NM-C and NP-AERD fibroblasts. Incubation with either exogenous PGE2 or selective EP2 agonist significantly increased expression of IL-1RI in NM-C fibroblasts and had hardly any effect on NP-AERD fibroblasts. Alterations in IL-1RI, COX-2, and mPGES-1 expression that were found in NP-AERD fibroblasts were corrected when EP2 receptor expression was normalized by transfection of NP-AERD fibroblasts. Conclusion Altered expression of EP2 in patients with AERD contributes to deficient induction of IL-1RI, reducing the capacity of IL-1β to increase COX-2 and mPGES-1 expression, which results in low PGE2 production. This impairment in the generation of PGE2 subsequently reduces its ability to induce IL-1RI.
    Original languageEnglish
    Pages (from-to)99-107.e7
    JournalJournal of Allergy and Clinical Immunology
    Volume137
    Issue number1
    DOIs
    Publication statusPublished - 1 Jan 2016

    Keywords

    • Aspirin-exacerbated respiratory disease
    • COX-2
    • E-prostanoid 2 receptor
    • fibroblasts
    • IL-1 receptor type I
    • IL-1β
    • microsomal prostaglandin E synthase 1
    • nasal mucosa
    • nasal polyps
    • prostaglandin E 2

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