Long-term evolution of acinar-to-ductal metaplasia and β-cell mass after radiofrequency-assisted transection of the pancreas in a controlled large animal model

Rita Quesada, Anna Andaluz, Marta Caceres, Xavier Moll, Mar Iglesias, Dimitri Dorcaratto, Ignasi Poves, Enrique Berjano, Luis Grande, Fernando Burdío

Research output: Contribution to journalArticleResearchpeer-review

3 Citations (Scopus)

Abstract

Copyright © 2015, IAP and EPC. Published by Elsevier India, a division of Reed Elsevier India Pvt. Ltd. All rights reserved. Background: Pancreatic duct ligation (PDL) has been used as a model of chronic pancreatitis and as a model to increase β-cell mass. However, studies in mice have demonstrated acinar regeneration after PDL, questioning the long-term validity of the model. We aim to elucidate whether RF-assisted transection (RFAT) of the main pancreatic duct is a reliable PDL model, both in short (ST, 1-month) and longterm (LT, 6-months) follow-ups. Methods: Eleven pigs were subjected to RFAT. Biochemical (serum/peripancreatic amylase and glucose) and histological changes (including a semiautomatic morphometric study of over 1000 images/pancreas and IHC analysis) were evaluated after ST or LT follow-up and also in fresh pancreas specimens that were used as controls for 1 (n = 4) and 6 months (n = 6). Results: The distal pancreas in the ST was characterized by areas of acinar-to-ductal metaplasia (56%) which were significantly reduced at LT (21%) by fibrotic replacement and adipose tissue. The endocrine mass showed a normal increase. Conclusion: RFAT in the pig seems to be an appropriate PDL model without restoration of pancreatic drainage or reduction of endocrine mass.
Original languageEnglish
Pages (from-to)38-43
JournalPancreatology
Volume16
Issue number1
DOIs
Publication statusPublished - 1 Jan 2016

Keywords

  • Acinar-to-ductal metaplasia
  • Animal model β-cell mass
  • Duodenopancreatectomy
  • Pancreatic duct ligation
  • Radiofrequency-assisted transection

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