Localization of Zinc and Cadmium in Thlaspi caerulescens (Brassicaceae), a Metallophyte that can Hyperaccumulate both Metals

M. D. Vázquez; J. Barceló; Ch Poschenrieder; J. Mádico; P. Hatton; A. J.M. Baker; G. H. Cope

doi:10.1016/S0176-1617(11)81091-6

Localization of Zinc and Cadmium in Thlaspi caerulescens (Brassicaceae), a Metallophyte that can Hyperaccumulate both Metals

M. D. Vázquez, J. Barceló, Ch Poschenrieder, J. Mádico, P. Hatton, A. J.M. Baker, G. H. Cope

Department of Animal Biology, Plant Biology and Ecology

Research output: Contribution to journal › Article › Research › peer-review

179 Citations (Scopus)

Abstract

The compartmentation of Zn and Cd in roots of Thlaspi caerulescens J. & C. Presl, a metallophyte that hyperaccumulates both metals, was investigated by means of EDAX. Two methods for sample preparation, Na2S-fixation and freeze-substitution, were compared. Na2S-fixation was not suitable for preventing the loss of metal ions from the samples. According to our results from freeze-substituted samples, Cd accumulated mainly in the apoplast and, to a lesser extent, in vacuoles, whereas Zn was principally found in vacuoles and, to a lesser extent, in cell walls. © 1992, Gustav Fischer Verlag, Stuttgart. All rights reserved.

Original language	English
Pages (from-to)	350-355
Journal	Journal of Plant Physiology
Volume	140
Issue number	3
DOIs	https://doi.org/10.1016/S0176-1617(11)81091-6
Publication status	Published - 1 Jan 1992

Keywords

cadmium
chemical fixation
freeze-substitution
metal hyperaccumulator
Thlaspi caerulescens
X-ray microanalysis
zinc

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title = "Localization of Zinc and Cadmium in Thlaspi caerulescens (Brassicaceae), a Metallophyte that can Hyperaccumulate both Metals",

abstract = "The compartmentation of Zn and Cd in roots of Thlaspi caerulescens J. & C. Presl, a metallophyte that hyperaccumulates both metals, was investigated by means of EDAX. Two methods for sample preparation, Na2S-fixation and freeze-substitution, were compared. Na2S-fixation was not suitable for preventing the loss of metal ions from the samples. According to our results from freeze-substituted samples, Cd accumulated mainly in the apoplast and, to a lesser extent, in vacuoles, whereas Zn was principally found in vacuoles and, to a lesser extent, in cell walls. {\textcopyright} 1992, Gustav Fischer Verlag, Stuttgart. All rights reserved.",

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Localization of Zinc and Cadmium in Thlaspi caerulescens (Brassicaceae), a Metallophyte that can Hyperaccumulate both Metals. / Vázquez, M. D.; Barceló, J.; Poschenrieder, Ch; Mádico, J.; Hatton, P.; Baker, A. J.M.; Cope, G. H.

In: Journal of Plant Physiology, Vol. 140, No. 3, 01.01.1992, p. 350-355.

Research output: Contribution to journal › Article › Research › peer-review

TY - JOUR

T1 - Localization of Zinc and Cadmium in Thlaspi caerulescens (Brassicaceae), a Metallophyte that can Hyperaccumulate both Metals

AU - Vázquez, M. D.

AU - Barceló, J.

AU - Poschenrieder, Ch

AU - Mádico, J.

AU - Hatton, P.

AU - Baker, A. J.M.

AU - Cope, G. H.

PY - 1992/1/1

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N2 - The compartmentation of Zn and Cd in roots of Thlaspi caerulescens J. & C. Presl, a metallophyte that hyperaccumulates both metals, was investigated by means of EDAX. Two methods for sample preparation, Na2S-fixation and freeze-substitution, were compared. Na2S-fixation was not suitable for preventing the loss of metal ions from the samples. According to our results from freeze-substituted samples, Cd accumulated mainly in the apoplast and, to a lesser extent, in vacuoles, whereas Zn was principally found in vacuoles and, to a lesser extent, in cell walls. © 1992, Gustav Fischer Verlag, Stuttgart. All rights reserved.

AB - The compartmentation of Zn and Cd in roots of Thlaspi caerulescens J. & C. Presl, a metallophyte that hyperaccumulates both metals, was investigated by means of EDAX. Two methods for sample preparation, Na2S-fixation and freeze-substitution, were compared. Na2S-fixation was not suitable for preventing the loss of metal ions from the samples. According to our results from freeze-substituted samples, Cd accumulated mainly in the apoplast and, to a lesser extent, in vacuoles, whereas Zn was principally found in vacuoles and, to a lesser extent, in cell walls. © 1992, Gustav Fischer Verlag, Stuttgart. All rights reserved.

KW - cadmium

KW - chemical fixation

KW - freeze-substitution

KW - metal hyperaccumulator

KW - Thlaspi caerulescens

KW - X-ray microanalysis

KW - zinc

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DO - 10.1016/S0176-1617(11)81091-6

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