Lipopolysaccharide-binding protein and soluble CD14 in the vitreous fluid of patients with proliferative diabetic retinopathy

Cristina Hernández, Francisco Ortega, Marta García-Ramírez, Marta Villarroel, Joan Casado, Luis García-Pascual, José Manuel Fernández-Real, Rafael Simó

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8 Citations (Scopus)

Abstract

Purpose: The purpose of this study was to compare intravitreous levels of lipopolysaccharide-binding protein and soluble CD14 (sCD14) between patients with proliferative diabetic retinopathy (PDR) and nondiabetic subjects. Methods: This study included 19 consecutive Type 2 diabetic patients with PDR in whom a vitrectomy was performed. Sixteen vitreous humors from nondiabetic patients matched by age, with idiopathic macular holes, were selected from our vitreous bank and used as a control group. Lipopolysaccharide-binding protein was assessed by enzyme-linked immunosorbent assay and sCD14 by a solid-phase enzyme-amplified sensitive immunoassay. Results: Lipopolysaccharide-binding protein and sCD14 levels were significantly higher in patients with PDR than in the control group (lipopolysaccharide-binding protein, P < 0.001; sCD14, P < 0.01). After correcting for vitreal proteins, the results remained significantly higher in patients with PDR. No differences in serum levels were observed, and we did not find any correlation between serum and vitreous levels. A direct correlation between lipopolysaccharide-binding protein and sCD14 was detected in the vitreous fluid (r = 0.57; P < 0.001) but not in the plasma. Finally, a significant correlation between intravitreal levels of both lipopolysaccharide-binding protein and sCD14 and interleukin-8 and monocyte chemotactic protein-1 was also detected. Conclusion: Lipopolysaccharide-binding protein and sCD14 are elevated in the vitreous fluid of patients with PDR and thus may play a role in the innate immune response triggered by the inflammatory injury characteristic of PDR. Copyright © by Ophthalmic Communications Society, Inc.
Original languageEnglish
Pages (from-to)345-352
JournalRetina
Volume30
Issue number2
DOIs
Publication statusPublished - 1 Feb 2010

Keywords

  • Diabetic retinopathy
  • Inflammation
  • Lipopolysaccharide-binding protein
  • Soluble CD14
  • Vitreous fluid

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