Extracellular lipase production by Candidarugosa growth has been studied. The main growth parameters, and the lipase activity in the culture broth were determined in order to identify the maximum of enzyme activity. The effect of lipidic material and size and growth phase of the inoculum on enzymatic production have been studied. Maximum extracellular lipase activity was associated with an increase in enzyme production when the number of viable cells started to decrease. © 1988 Kluwer Academic Publishers.