TY - JOUR
T1 - Limited Emergence of Salmonella enterica Serovar Infantis Variants with Reduced Phage Susceptibility in PhagoVet-Treated Broilers
AU - Sevilla-Navarro, Sandra
AU - Otero, Jennifer
AU - López-Pérez, Júlia
AU - Torres-Boncompte, Jan
AU - Prucha, Tiago
AU - Gussem, Maarten De
AU - Silva, Daniela
AU - Burgan, Julia
AU - Catalá-Gregori, Pablo
AU - Cortés, Pilar
AU - Llagostera, Montserrat
N1 - Publisher Copyright:
© 2024 by the authors.
PY - 2024/8/14
Y1 - 2024/8/14
N2 - Salmonella enterica serovar Infantis (S. Infantis) poses a growing issue in the poultry sector, with phage-based products emerging as a safe and effective control measure. This study investigated the emergence of reduced-phage-susceptibility variants (RPSV) of S. Infantis in PhagoVet-treated broilers, given that RPSV could undermine phage treatment efficacy. The bacteriophages in the PhagoVet product were characterized using transmission electron microscopy (TEM), genome sequencing, and infection profiling. Furthermore, two broiler trials were conducted: a challenge group (T1) and a challenge-and-treated group (T2). The S. Infantis infective dose was set at 10
4 and 10
6 colony-forming units (CFUs) per animal, with PhagoVet administration at 10
6 and 10
8 plaque-forming units (PFUs) per animal, in Trials 1 and 2, respectively. The results revealed that the four PhagoVet bacteriophages belonged to different genera. PhagoVet evidenced broad-spectrum efficacy against 271 strains representing 18 Salmonella serovars. In Trial 1, PhagoVet reduced bacterial counts in feces to nearly undetectable levels by day 42, with no RPSV detected. However, in Trial 2, three and five RPSVs were detected in feces and ceca, respectively. Consequently, PhagoVet demonstrated efficacy against S. Infantis in broilers, and the potential impact of RPSV is deemed unlikely to compromise its efficacy.
AB - Salmonella enterica serovar Infantis (S. Infantis) poses a growing issue in the poultry sector, with phage-based products emerging as a safe and effective control measure. This study investigated the emergence of reduced-phage-susceptibility variants (RPSV) of S. Infantis in PhagoVet-treated broilers, given that RPSV could undermine phage treatment efficacy. The bacteriophages in the PhagoVet product were characterized using transmission electron microscopy (TEM), genome sequencing, and infection profiling. Furthermore, two broiler trials were conducted: a challenge group (T1) and a challenge-and-treated group (T2). The S. Infantis infective dose was set at 10
4 and 10
6 colony-forming units (CFUs) per animal, with PhagoVet administration at 10
6 and 10
8 plaque-forming units (PFUs) per animal, in Trials 1 and 2, respectively. The results revealed that the four PhagoVet bacteriophages belonged to different genera. PhagoVet evidenced broad-spectrum efficacy against 271 strains representing 18 Salmonella serovars. In Trial 1, PhagoVet reduced bacterial counts in feces to nearly undetectable levels by day 42, with no RPSV detected. However, in Trial 2, three and five RPSVs were detected in feces and ceca, respectively. Consequently, PhagoVet demonstrated efficacy against S. Infantis in broilers, and the potential impact of RPSV is deemed unlikely to compromise its efficacy.
KW - PhagoVet
KW - Salmonella Infantis
KW - bacteriophage
KW - broilers
KW - reduced phage susceptibility
UR - http://www.scopus.com/inward/record.url?scp=85202585821&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/76fdbc90-e432-3135-a515-2d6da4d02bb2/
U2 - 10.3390/ani14162352
DO - 10.3390/ani14162352
M3 - Article
C2 - 39199885
SN - 2076-2615
VL - 14
JO - Animals
JF - Animals
IS - 16
M1 - 2352
ER -