The use of human Peripheral Blood Mononuclear Cells (PBMC) for research purposes is widely extended. Typically, PBMC are separated from blood donations after volume reduction (buffy coat) and a subsequent density gradient centrifugation. However, policies on blood donation and processing for transfusion medicine have introduced the use of Leukocyte Reduction Filters (LRF). Blood Transfusion Services still process blood samples and produce buffy coats, but only a few units and specifically to obtain random platelets. Therefore, it is important to find alternative sources of PBMC. We have tested the possibility to use the cells retained by the LRF as an alternative source, with special interest on monocytes, because of their potential use as dendritic cell (DC) precursors for immunotherapy. Cells from filters were recovered at high numbers (about 5×108 PBMC) and viability (> 95%), and the phenotypic and functional experiments confirmed that they were similar to those cells obtained from buffy coats. Monocytes were differentiated in vitro to DC, showing no differences from those obtained from the buffy coats. The results have shown that elution of PBMC from LRF is a valid alternative source to obtain all blood cell types, including monocytes to generate functional DC.
|Publication status||Published - 1 Jul 2003|
- Dendritic cells
- Leukocyte reduction
- Mononuclear cells