Lethality and injuring the effect of compression and decompression rates of high hydrostatic pressure on Escherichia coli O157:H7 in different matrices

Qamar Abbas Syed, Martin Buffa, Buenaventura Guamis, Jordi Saldo

Research output: Contribution to journalArticleResearchpeer-review

21 Citations (Scopus)

Abstract

The effect of compression and decompression rates of high hydrostatic pressure (HHP) on Escherichia coli O157:H7 was investigated. Samples of orange juice, skimmed milk and Tris buffer were inoculated with E. coli O157:H7 and subjected to 600 MPa for 3 min at 4°C with fast, medium and slow compression and decompression. Analyses immediately after HHP treatment revealed that E. coli in milk and juice treated with fast compression suffered more than slow compression rates. Slow decompression resulted in higher inactivation of E. coli in all matrices. After overnight storage, highest stress-recovery (1.19 log cfu/mL) was observed in Tris buffer. Healthy cells were < 1 log cfu/mL in milk and buffer samples, but no growth was detected in orange juice for any of the treatments immediately after HHP. After 15 days at 4°C, E. coli cells in skimmed milk and Tris buffer recovered significantly, whereas the recovery of sublethally injured cells was inhibited in orange juice. © 2013 Copyright Taylor and Francis Group, LLC.
Original languageEnglish
Pages (from-to)64-72
JournalHigh Pressure Research
Volume33
Issue number1
DOIs
Publication statusPublished - 1 Mar 2013

Keywords

  • Compression rate
  • Decompression rate
  • E. coli
  • High hydrostatic pressure

Fingerprint Dive into the research topics of 'Lethality and injuring the effect of compression and decompression rates of high hydrostatic pressure on Escherichia coli O157:H7 in different matrices'. Together they form a unique fingerprint.

Cite this