Lanthanide modulation of the orientation of macromolecules induced by purple membrane

Pau Bernadó, Renato Barbieri, Esteve Padrós, Claudio Luchinat, Miquel Pons

Research output: Contribution to journalArticleResearchpeer-review

9 Citations (Scopus)

Abstract

Addition of Tb3+ to purple membrane (PM) suspensions changes the orientation of the menbrane normal from parallel to perpendicular with respect to the magnetic field. Residual dipolar couplings measured in protein L in the presence of PM are scaled by a factor of -1/2. NMR line broadening and cross-correlation effects induced by the addition of PM are partially reversed by Tb3+ but not by Tm3+ that has no effect on the orientation of PM. This is interpreted as the result of anisotropically restricted motion of protein L in the transiently PM-bound form. Copyright © 2002 American Chemical Society.
Original languageEnglish
Pages (from-to)374-375
JournalJournal of the American Chemical Society
Volume124
DOIs
Publication statusPublished - 23 Jan 2002

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