Lack of PrPsc immunostaining in intracranial ectopic lymphoid follicles in a sheep with concomitant non-suppurative encephalitis and Nor98-like atypical scrapie: A case report: A case report

E. Vidal, R. Tortosa, C. Costa, J. Benavides, O. Francino, E. Sánchez-Robert, V. Pérez, M. Pumarola*

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

9 Citations (Scopus)

Abstract

During active surveillance for transmissible spongiform encephalopathies (TSEs) in sheep, an initial reactor was detected using a rapid test on a brain sample. Immunohistochemistry confirmed an atypical TSE presentation that closely resembled the previously described Nor98 cases. Sequencing of the prnp gene confirmed the ARQ/AHQ genotype with the L141F mutation at codon 141 associated with this phenotype. The head, including the brain and cranial lymphoid tissues, was sampled and examined thoroughly. Non-purulent encephalitis, with ectopic lymphoid follicle formation within the brain, was diagnosed concomitant to the TSE. When scrapie-associated prion protein (PrPsc) deposition was studied by immunohistochemistry there was a noticeable lack of lymphotropism. The distribution of PrPsc in the brain differed considerably from that of classical scrapie cases. Astrogliosis and microgliosis were demonstrated by histochemical procedures.

Original languageEnglish
Pages (from-to)283-288
Number of pages6
JournalVeterinary Journal
Volume177
Issue number2
DOIs
Publication statusPublished - 1 Aug 2008

Keywords

  • Atypical scrapie
  • Lymphotropism
  • Ovine
  • PrP sc

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