Label-free Bacteria Quantification in Blood Plasma by a Bioprinted Microarray Based Interferometric Point-of-Care Device

Priyanka Dey, Nuria Fabri-Faja, Olalla Calvo-Lozano, Roland A. Terborg, Alexander Belushkin, Filiz Yesilkoy, Anna Fàbrega, Juan Carlos Ruiz-Rodriguez, Ricard Ferrer, Juan José González-López, Maria Carmen Estévez, Hatice Altug, Valerio Pruneri, Laura M. Lechuga

Research output: Contribution to journalArticleResearch

19 Citations (Scopus)

Abstract

© 2018 American Chemical Society. Existing clinical methods for bacteria detection lack speed, sensitivity, and, importantly, point-of-care (PoC) applicability. Thus, finding ways to push the sensitivity of clinical PoC biosensing technologies is crucial. Here we report a portable PoC device based on lens-free interferometric microscopy (LIM). The device employs high performance nanoplasmonics and custom bioprinted microarrays and is capable of direct label-free bacteria (E. coli) quantification. With only one-step sample handling we offer a sample-to-data turnaround time of 40 min. Our technology features detection sensitivity of a single bacterial cell both in buffer and in diluted blood plasma and is intrinsically limited by the number of cells present in the detection volume. When employed in a hospital setting, the device has enabled accurate categorization of sepsis patients (infectious SIRS) from control groups (healthy individuals and noninfectious SIRS patients) without false positives/negatives. User-friendly on-site bacterial clinical diagnosis can thus become a reality.
Original languageEnglish
Pages (from-to)52-60
JournalACS Sensors
Volume4
DOIs
Publication statusPublished - 25 Jan 2019

Keywords

  • bacteria
  • label-free detection
  • microarray
  • nanoplasmonics
  • plasma samples
  • sepsis

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