Kinetics of xylem loading, membrane potential maintenance, and sensitivity of K + -permeable channels to reactive oxygen species: Physiological traits that differentiate salinity tolerance between pea and barley

Jayakumar Bose, Lana Shabala, Igor Pottosin, Fanrong Zeng, Ana Maria Velarde-Buendía, Amandine Massart, Charlotte Poschenrieder, Yuda Hariadi, Sergey Shabala

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78 Citations (Scopus)

Abstract

Salt sensitive (pea) and salt tolerant (barley) species were used to understand the physiological basis of differential salinity tolerance in crops. Pea plants were much more efficient in restoring otherwise depolarized membrane potential thereby effectively decreasing K + efflux through depolarization-activated outward rectifying potassium channels. At the same time, pea root apex was 10-fold more sensitive to physiologically relevant H 2 O 2 concentration and accumulated larger amounts of H 2 O 2 under saline conditions. This resulted in a rapid loss of cell viability in the pea root apex. Barley plants rapidly loaded Na + into the xylem; this increase was only transient, and xylem and leaf Na + concentration remained at a steady level for weeks. On the contrary, pea plants restricted xylem Na + loading during the first few days of treatment but failed to prevent shoot Na + elevation in the long term. It is concluded that superior salinity tolerance of barley plants compared with pea is conferred by at least three different mechanisms: (1) efficient control of xylem Na + loading; (2) efficient control of H 2 O 2 accumulation and reduced sensitivity of non-selective cation channels to H 2 O 2 in the root apex; and (3) higher energy saving efficiency, with less ATP spent to maintain membrane potential under saline conditions. Salinity tolerance is a complex physiological trait composed of multiple sub-traits. Here we reveal that efficient control of xylem Na + loading, control of H 2 O 2 accumulation, reduced sensitivity of non-selective cation channels to H 2 O 2 in the root apex, and higher energy saving efficiency are the major physiological traits distinguishing between salt-tolerant (barley) and salt-sensitive (pea) crop species. These traits should be targeted in plant breeding programs aimed on improving salinity tolerance via "pyramiding approach". © 2014 John Wiley & Sons Ltd.
Original languageEnglish
Pages (from-to)589-600
JournalPlant, Cell and Environment
Volume37
DOIs
Publication statusPublished - 1 Mar 2014

Keywords

  • H -ATPase +
  • Ion channels
  • Membrane depolarization
  • Potassium homeostasis

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