Keratin expression in normal vulva, non-neoplastic epithelial disorders, vulvar intraepithelial neoplasia, and invasive squamous cell carcinoma

Jordi Esquius, Massimo Brisigotti, Xavier Matias-Guiu, Jaime Prat

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11 Citations (Scopus)

Abstract

The expression of AE1, AE2, AE3. and CAM 5.2 antikeratin monoclonal antibodies was investigated in 68 vulvar specimens by an avidin-biotin complex (ABC) method. They included normal vulva (NV. 10). non-neoplastic epithelial disorders (NNED, 31), vulvar intraepithelial neoplasia (VIN, 17), and squamous cell carcinoma (SCC. 10). AEI weakly stained the basal cell layer in NV, exhibited a uniform suprabasal stain in hyperplasia, failed to stain dysplastic areas in VIN I-II, and was patchy and disorganized in VIN III and SCC. AE2 stained the upper third of the epithelium in NV, NNED, and VIN I-II, but it failed to stain VIN III basaloid and SCC; VIN III bowenoid was locally positive. AE3 offered little information, because it stained all lesions: VIN III and SCC. however, exhibited a patchy and disorganized stain. CAM 5.2 was expressed in only half of the cases of VIN III basaloid and in one case each of VIN I-II and SCC. We conclude that keratin expression varies according to the degree of dysplasia: AEI may serve to separate certain cases of NNED from VIN I-II: AE2 and CAM 5.2 are useful to differentiate both histologic types of VIN III. © 1991 International Society of Gynecological Pathologists.
Original languageEnglish
Pages (from-to)341-355
JournalInternational Journal of Gynecological Pathology
Volume10
Issue number4
DOIs
Publication statusPublished - 1 Jan 1991

Keywords

  • Basal cell carcinoma
  • Monodermal neoplasia
  • Ovarian dermoid cysts
  • Sebaceous tumors

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