TY - JOUR
T1 - Karyotyping of human synaptonemal complexes by cenM-FISH
AU - Oliver-Bonet, Maria
AU - Liehr, Thomas
AU - Nietzel, Angela
AU - Heller, Anita
AU - Starke, Heike
AU - Claussen, Uwe
AU - Codina-Pascual, Montserrat
AU - Pujol, Aïda
AU - Abad, Carlos
AU - Egozcue, Josep
AU - Navarro, Joaquima
AU - Benet, Jordi
PY - 2003/11/1
Y1 - 2003/11/1
N2 - The purpose of this work was to adapt the recently described centromere-specific multicolour (cenM-) FISH technique to human meiotic cells, and evaluate the usefulness of this multiplex fluorescence method for karyotyping human synaptonemal complex (SC), previously analysed by immunocytogenetic approaches. The results obtained demonstrate that cenM-FISH is a reliable one-single-step method, which allows for the identification of all SC present in pachytene spreads. Moreover, when cenM-FISH is applied after immunocytogenetic analysis, the number and distribution of MLH1 foci per chromosome can be established and recombination analysis for each chromosome can be performed easily.
AB - The purpose of this work was to adapt the recently described centromere-specific multicolour (cenM-) FISH technique to human meiotic cells, and evaluate the usefulness of this multiplex fluorescence method for karyotyping human synaptonemal complex (SC), previously analysed by immunocytogenetic approaches. The results obtained demonstrate that cenM-FISH is a reliable one-single-step method, which allows for the identification of all SC present in pachytene spreads. Moreover, when cenM-FISH is applied after immunocytogenetic analysis, the number and distribution of MLH1 foci per chromosome can be established and recombination analysis for each chromosome can be performed easily.
KW - cenM-FISH on synaptonemal complexes
KW - Immunocytogenetics
KW - Meiotic recombination
U2 - 10.1038/sj.ejhg.5201067
DO - 10.1038/sj.ejhg.5201067
M3 - Article
VL - 11
SP - 879
EP - 883
JO - European Journal of Human Genetics
JF - European Journal of Human Genetics
SN - 1018-4813
IS - 11
ER -