Karyotyping of human synaptonemal complexes by cenM-FISH

Maria Oliver-Bonet, Thomas Liehr, Angela Nietzel, Anita Heller, Heike Starke, Uwe Claussen, Montserrat Codina-Pascual, Aïda Pujol, Carlos Abad, Josep Egozcue, Joaquima Navarro, Jordi Benet

Research output: Contribution to journalArticleResearchpeer-review

32 Citations (Scopus)

Abstract

The purpose of this work was to adapt the recently described centromere-specific multicolour (cenM-) FISH technique to human meiotic cells, and evaluate the usefulness of this multiplex fluorescence method for karyotyping human synaptonemal complex (SC), previously analysed by immunocytogenetic approaches. The results obtained demonstrate that cenM-FISH is a reliable one-single-step method, which allows for the identification of all SC present in pachytene spreads. Moreover, when cenM-FISH is applied after immunocytogenetic analysis, the number and distribution of MLH1 foci per chromosome can be established and recombination analysis for each chromosome can be performed easily.
Original languageEnglish
Pages (from-to)879-883
JournalEuropean Journal of Human Genetics
Volume11
Issue number11
DOIs
Publication statusPublished - 1 Nov 2003

Keywords

  • cenM-FISH on synaptonemal complexes
  • Immunocytogenetics
  • Meiotic recombination

Fingerprint Dive into the research topics of 'Karyotyping of human synaptonemal complexes by cenM-FISH'. Together they form a unique fingerprint.

Cite this