Karyotyping of human synaptonemal complexes by cenM-FISH

Maria Oliver-Bonet; Thomas Liehr; Angela Nietzel; Anita Heller; Heike Starke; Uwe Claussen; Montserrat Codina-Pascual; Aïda Pujol; Carlos Abad; Josep Egozcue; Joaquima Navarro; Jordi Benet

doi:10.1038/sj.ejhg.5201067

Karyotyping of human synaptonemal complexes by cenM-FISH

Maria Oliver-Bonet, Thomas Liehr, Angela Nietzel, Anita Heller, Heike Starke, Uwe Claussen, Montserrat Codina-Pascual, Aïda Pujol, Carlos Abad, Josep Egozcue, Joaquima Navarro, Jordi Benet

Research output: Contribution to journal › Article › Research › peer-review

32 Citations (Scopus)

Abstract

The purpose of this work was to adapt the recently described centromere-specific multicolour (cenM-) FISH technique to human meiotic cells, and evaluate the usefulness of this multiplex fluorescence method for karyotyping human synaptonemal complex (SC), previously analysed by immunocytogenetic approaches. The results obtained demonstrate that cenM-FISH is a reliable one-single-step method, which allows for the identification of all SC present in pachytene spreads. Moreover, when cenM-FISH is applied after immunocytogenetic analysis, the number and distribution of MLH1 foci per chromosome can be established and recombination analysis for each chromosome can be performed easily.

Original language	English
Pages (from-to)	879-883
Journal	European Journal of Human Genetics
Volume	11
Issue number	11
DOIs	https://doi.org/10.1038/sj.ejhg.5201067
Publication status	Published - 1 Nov 2003

Keywords

cenM-FISH on synaptonemal complexes
Immunocytogenetics
Meiotic recombination

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title = "Karyotyping of human synaptonemal complexes by cenM-FISH",

abstract = "The purpose of this work was to adapt the recently described centromere-specific multicolour (cenM-) FISH technique to human meiotic cells, and evaluate the usefulness of this multiplex fluorescence method for karyotyping human synaptonemal complex (SC), previously analysed by immunocytogenetic approaches. The results obtained demonstrate that cenM-FISH is a reliable one-single-step method, which allows for the identification of all SC present in pachytene spreads. Moreover, when cenM-FISH is applied after immunocytogenetic analysis, the number and distribution of MLH1 foci per chromosome can be established and recombination analysis for each chromosome can be performed easily.",

keywords = "cenM-FISH on synaptonemal complexes, Immunocytogenetics, Meiotic recombination",

author = "Maria Oliver-Bonet and Thomas Liehr and Angela Nietzel and Anita Heller and Heike Starke and Uwe Claussen and Montserrat Codina-Pascual and A{\"i}da Pujol and Carlos Abad and Josep Egozcue and Joaquima Navarro and Jordi Benet",

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doi = "10.1038/sj.ejhg.5201067",

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Karyotyping of human synaptonemal complexes by cenM-FISH. / Oliver-Bonet, Maria; Liehr, Thomas; Nietzel, Angela; Heller, Anita; Starke, Heike; Claussen, Uwe; Codina-Pascual, Montserrat; Pujol, Aïda; Abad, Carlos; Egozcue, Josep; Navarro, Joaquima ; Benet, Jordi.

In: European Journal of Human Genetics, Vol. 11, No. 11, 01.11.2003, p. 879-883.

Research output: Contribution to journal › Article › Research › peer-review

TY - JOUR

T1 - Karyotyping of human synaptonemal complexes by cenM-FISH

AU - Oliver-Bonet, Maria

AU - Liehr, Thomas

AU - Nietzel, Angela

AU - Heller, Anita

AU - Starke, Heike

AU - Claussen, Uwe

AU - Codina-Pascual, Montserrat

AU - Pujol, Aïda

AU - Abad, Carlos

AU - Egozcue, Josep

AU - Navarro, Joaquima

AU - Benet, Jordi

PY - 2003/11/1

Y1 - 2003/11/1

N2 - The purpose of this work was to adapt the recently described centromere-specific multicolour (cenM-) FISH technique to human meiotic cells, and evaluate the usefulness of this multiplex fluorescence method for karyotyping human synaptonemal complex (SC), previously analysed by immunocytogenetic approaches. The results obtained demonstrate that cenM-FISH is a reliable one-single-step method, which allows for the identification of all SC present in pachytene spreads. Moreover, when cenM-FISH is applied after immunocytogenetic analysis, the number and distribution of MLH1 foci per chromosome can be established and recombination analysis for each chromosome can be performed easily.

AB - The purpose of this work was to adapt the recently described centromere-specific multicolour (cenM-) FISH technique to human meiotic cells, and evaluate the usefulness of this multiplex fluorescence method for karyotyping human synaptonemal complex (SC), previously analysed by immunocytogenetic approaches. The results obtained demonstrate that cenM-FISH is a reliable one-single-step method, which allows for the identification of all SC present in pachytene spreads. Moreover, when cenM-FISH is applied after immunocytogenetic analysis, the number and distribution of MLH1 foci per chromosome can be established and recombination analysis for each chromosome can be performed easily.

KW - cenM-FISH on synaptonemal complexes

KW - Immunocytogenetics

KW - Meiotic recombination

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DO - 10.1038/sj.ejhg.5201067

M3 - Article

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EP - 883

JO - European Journal of Human Genetics

JF - European Journal of Human Genetics

SN - 1018-4813

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