Thalassocella blandensis gen. Nov., sp. nov., a novel member of the family Cellvibrionaceae

Teresa Lucena, David R. Arahal, Isabel Sanz-Sáez, Silvia G. Acinas, Olga Sánchez, Rosa Aznar, Carlos Pedrós-Alió, María J. Pujalte*

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

6 Citations (Scopus)

Abstract

Strain ISS155(T), isolated from surface Mediterranean seawater, has cells that are Gram-reaction-negative, motile, strictly aerobic chemoorganotrophic, oxidase-positive, unable to reduce nitrate to nitrite, and able to grow with cellulose as the sole carbon and energy source. It is mesophilic, neutrophilic, slightly halophilic and has a requirement for sodium and magnesium ions. Its 16S rRNA gene sequence places the strain among members of Cellvibrionaceae, in the Gammaproteobacteria, with Agarilytica rhodophyticola 017(T) as closest relative (94.3% similarity). Its major cellular fatty acids are C18.1C16:0 and C-16:1;major phospholipids are phosphatidyl glycerol, phosphatidyl ethanolamine and an unidentified lipid, and the major respiratory quinone is Q8. The genome size is 6.09 Mbp and G+C content is 45.2 mol%. A phylogenomic analysis using UBCG merges strain ISS155(T) in a Glade with A. rhodophyticola, Teredinibacter turnerae. Saccharophagus degradans and Agaribacterium haliotis type strain genomes, all of them possessing a varied array of carbohydrate-active enzymes and the potential for polysaccharide degradation. Average amino acid identity indexes determined against available Cellvibrionaceae type strain genomes show that strain ISS155(T) is related to them by values lower than 60%, with a maximum of 58% to A. rhodophyticola 017(T) and 57% to T. turnerae T7902(T) and S. degradans 2-40(T). These results, together with the low 165 rRNA gene sequence similarities and differences in phenotypic profiles, indicate that strain ISS155(T) represents a new genus and species in Cellvibrionaceae, for which we propose the name Thalassocella blandensis gen. nov., sp. nov.. and strain ISS155(T) (=CECT 9533(T)=LMG 3123(T)) as the type strain.

Original languageAmerican English
Article number003906
Pages (from-to)1231-1239
Number of pages9
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume70
Issue number2
DOIs
Publication statusPublished - 1 Feb 2020

Keywords

  • AAI
  • Agarilytica
  • ANI
  • Cellvibrionaceae
  • Marine bacteria
  • Teredinibacter
  • Thalassocella

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