Irreversible electroporation of the liver: Is there a safe limit to the ablation volume?

P. Sánchez-Velázquez, Q. Castellví, A. Villanueva, R. Quesada, C. Pañella, M. Cáceres, D. Dorcaratto, A. Andaluz, X. Moll, M. Trujillo, J. M. Burdió, E. Berjano, L. Grande, A. Ivorra, F. Burdió

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Abstract

Irreversible electroporation is a fast-growing liver ablation technique. Although safety has been well documented in small ablations, our aim is to assess its safety and feasibility when a large portion of liver is ablated. Eighty-seven mice were subjected to high voltage pulses directly delivered across parallel plate electrodes comprising around 40% of mouse liver. One group consisted in 55 athymic-nude, in which a tumor from the KM12C cell line was grown and the other thirty-two C57-Bl6 non-tumoral mice. Both groups were subsequently divided into subsets according to the delivered field strength (1000 V/cm, 2000 V/cm) and whether or not they received anti-hyperkalemia therapy. Early mortality (less than 24 hours post-IRE) in the 2000 V/cm group was observed and revealed considerably higher mean potassium levels. In contrast, the animals subjected to a 2000 V/cm field treated with the anti-hyperkalemia therapy had higher survival rates (OR = 0.1, 95%CI = 0.02-0.32, p < 0.001). Early mortality also depended on the electric field magnitude of the IRE protocol, as mice given 1000 V/cm survived longer than those given 2000 V/cm (OR = 4.7, 95%CI = 1.8-11.8, p = 0.001). Our findings suggest that ionic disturbances, mainly due to potassium alterations, should be warned and envisioned when large volume ablations are performed by IRE.
Original languageEnglish
Article number23781
Pages (from-to)-
JournalScientific Reports
Volume1
Issue number6
DOIs
Publication statusPublished - 1 Apr 2016

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    Sánchez-Velázquez, P., Castellví, Q., Villanueva, A., Quesada, R., Pañella, C., Cáceres, M., Dorcaratto, D., Andaluz, A., Moll, X., Trujillo, M., Burdió, J. M., Berjano, E., Grande, L., Ivorra, A., & Burdió, F. (2016). Irreversible electroporation of the liver: Is there a safe limit to the ablation volume? Scientific Reports, 1(6), -. [23781]. https://doi.org/10.1038/srep23781, https://doi.org/10.1001/2013.jamasurg.100