Iron overload, measured as serum ferritin, increases brain damage induced by focal ischemia and early reperfusion

Isaac García-Yébenes, Mónica Sobrado, Ana Moraga, Juan G. Zarruk, Victor G. Romera, Jesús M. Pradillo, Natalia Perez De La Ossa, María A. Moro, Antoni Dávalos, Ignacio Lizasoain

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Abstract

High levels of iron, measured as serum ferritin, are associated to a worse outcome after stroke. However, it is not known whether ischemic damage might increase ferritin levels as an acute phase protein or whether iron overload affects stroke outcome. The objectives are to study the effect of stroke on serum ferritin and the contribution of iron overload to ischemic damage. Swiss mice were fed with a standard diet or with a diet supplemented with 2.5% carbonyl iron to produce iron overload. Mice were submitted to permanent (by ligature and by in situ thromboembolic models) or transient focal ischemia (by ligature for 1 or 3 h). Treatment with iron diet produced an increase in the basal levels of ferritin in all the groups. However, serum ferritin did not change after ischemia. Animals submitted to permanent ischemia had the same infarct volume in the groups studied. However, in mice submitted to transient ischemia followed by early (1 h) but not late reperfusion (3 h), iron overload increased ischemic damage and haemorrhagic transformation. Iron worsens ischemic damage induced by transient ischemia and early reperfusion. In addition, ferritin is a good indicator of body iron levels but not an acute phase protein after ischemia. © 2012 Elsevier Ltd. All rights reserved.
Original languageEnglish
Pages (from-to)1364-1369
JournalNeurochemistry International
Volume61
Issue number8
DOIs
Publication statusPublished - 1 Dec 2012

Keywords

  • Blood-brain barrier
  • Edema
  • Haemorrhagic transformation
  • Mice
  • Thrombin

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    García-Yébenes, I., Sobrado, M., Moraga, A., Zarruk, J. G., Romera, V. G., Pradillo, J. M., Perez De La Ossa, N., Moro, M. A., Dávalos, A., & Lizasoain, I. (2012). Iron overload, measured as serum ferritin, increases brain damage induced by focal ischemia and early reperfusion. Neurochemistry International, 61(8), 1364-1369. https://doi.org/10.1016/j.neuint.2012.09.014