IP-10 is an accurate biomarker for the diagnosis of tuberculosis in children

I. Latorre, J. Díaz, I. Mialdea, M. Serra-Vidal, N. Altet, C. Prat, N. Díez, A. Escribano, I. Casas, C. Rodrigo, V. Ausina, M. Ruhwald, J. Domínguez

Research output: Contribution to journalArticleResearchpeer-review

34 Citations (Scopus)

Abstract

© 2014 The British Infection Association. Objective: Performance of IFN-γ assays in children is compromised. Therefore, we investigated the utility of IP-10 for the detection of active tuberculosis (TB) and latent tuberculosis infection (LTBI) diagnosis in children; comparing its positivity with QuantiFERON-TB Gold In-Tube (QFN-G-IT) and T-SPOT.TB. Methods: We studied 230 children from three groups: active TB, screening (healthy children without known exposure to active TB patient screened at school or by their paediatrician) and contact-tracing studies. IFN-γ release was determined by QFN-G-IT and T-SPOT.TB. IP-10 was detected in QFN-G-IT supernatants by ELISA. Results: When combining QFN-G-IT and IP-10 assays, positive results improved significantly from 38.3% in QFN-G-IT and 33.9% in IP-10 to 41.3%. Age and type of contact were significant risk factors associated with positive QFN-G-IT and IP-10 results. IP-10 levels after antigen-specific stimulation were significantly higher in comparison to IFN-γ levels. Correlation between the three assays was good (. κ=0.717-0.783). Conclusions: IP-10 cytokine is expressed in response to TB specific-antigens used in QFN-G-IT. In conclusion, the use of IFN-γ T-cell based assays in combination with an additional IP-10 assay detection could be useful for diagnosing active TB and LTBI in children.
Original languageEnglish
Pages (from-to)590-599
JournalJournal of Infection
Volume69
Issue number6
DOIs
Publication statusPublished - 1 Jan 2014

Keywords

  • Children
  • IFN-γ
  • IP-10
  • Tuberculosis

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