Abstract
Rat striatal slices were incubated 1 hour in Krebs buffer with carbogen by continuos perfusion. When both MAO-B inhibitors PF9601N and l-deprenyl were added in the incubation medium, at 200 μM concentration, a diminution in the dopamine content was observed. The decrease in dopamine content was partially calcium dependent and showed a different mechanism between both compounds. When 1 mM concentration EGTA was added at the Krebs incubation medium without calcium, dopamine content was partially recovered, being not affected in case of l-deprenyl. When 50 μM dantrolene (a calcium vesicular release inhibitor) was added in the Krebs incubation medium without calcium, dopamine was partially recovered for l-deprenyl, being not affected in case of PF9601N. These data indicate that dopamine release is extracellular calcium dependent in case of PF9601N, whereas l-deprenyl depends on calcium intracellular origin.
Original language | English |
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Pages (from-to) | 237-242 |
Journal | Neurobiology |
Volume | 8 |
Issue number | 3-4 |
Publication status | Published - 1 Dec 2000 |
Keywords
- Antioxidative
- MAO-B inhibitor
- Neuroprotection