Involvement of CA2+ in dopamine release in striatal rat slices by PF9601N and 1-deprenyl

V. Pérez, M. Pastó, M. Unzeta

Research output: Contribution to journalArticleResearchpeer-review

2 Citations (Scopus)

Abstract

Rat striatal slices were incubated 1 hour in Krebs buffer with carbogen by continuos perfusion. When both MAO-B inhibitors PF9601N and l-deprenyl were added in the incubation medium, at 200 μM concentration, a diminution in the dopamine content was observed. The decrease in dopamine content was partially calcium dependent and showed a different mechanism between both compounds. When 1 mM concentration EGTA was added at the Krebs incubation medium without calcium, dopamine content was partially recovered, being not affected in case of l-deprenyl. When 50 μM dantrolene (a calcium vesicular release inhibitor) was added in the Krebs incubation medium without calcium, dopamine was partially recovered for l-deprenyl, being not affected in case of PF9601N. These data indicate that dopamine release is extracellular calcium dependent in case of PF9601N, whereas l-deprenyl depends on calcium intracellular origin.
Original languageEnglish
Pages (from-to)237-242
JournalNeurobiology
Volume8
Issue number3-4
Publication statusPublished - 1 Dec 2000

Keywords

  • Antioxidative
  • MAO-B inhibitor
  • Neuroprotection

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