Intragranulomatous necrosis in pulmonary granulomas is not related to resistance against Mycobacterium tuberculosis infection in experimental murine models induced by aerosol

Evelyn Guirado, Sergi Gordillo, Olga Gil, Jorge Díaz, Gustavo Tapia, Cristina Vilaplana, Vicenç Ausina, Pere Joan Cardona

Research output: Contribution to journalArticleResearchpeer-review

13 Citations (Scopus)

Abstract

Intragranulomatous necrosis is a primary feature in the natural history of human tuberculosis (TB). Unfortunately, this phenomenon is not usually seen in the experimental TB murine model. Artificial induction of this necrosis in pulmonary granulomas (INPG) may be achieved through aerosol inoculation of lipopolysaccharide (LPS) 3 weeks after Mycobacterium tuberculosis infection. At week 9 post-infection, the centre of primary granulomas became larger, showing eosinophilic necrosis. Interestingly, INPG induction was related to mice strains C57BL/6 and 129/Sv, but not to BALB/c and DBA/2. Furthermore, the same pattern was obtained with the induction of infection using a clinical M. tuberculosis strain (UTE 0335R) that naturally induces INPG. In all the mice strains tested, the study of pulmonary mRNA expression revealed a tendency to increase or to maintain the expression of RANTES, interferon-γ, tumour necrosis factor and iNOS, in both LPS- and UTE 0335R-induced INPG, thus suggesting that this response must be necessary but not sufficient for inducing INPG. Our work supports that INPG induction is a local phenomenon unrelated to the resistant (C57BL/6 and BALB/c) or susceptible (129/Sv and DBA/2) background of mice strains against M. tuberculosis infection. © 2006 Blackwell Publishing Ltd.
Original languageEnglish
Pages (from-to)139-149
JournalInternational Journal of Experimental Pathology
Volume87
Issue number2
DOIs
Publication statusPublished - 1 Apr 2006

Keywords

  • Intragranulomatous necrosis
  • Koch's reaction
  • Murine model
  • Mycobacterium tuberculosis
  • Shwartzman reaction

Fingerprint

Dive into the research topics of 'Intragranulomatous necrosis in pulmonary granulomas is not related to resistance against Mycobacterium tuberculosis infection in experimental murine models induced by aerosol'. Together they form a unique fingerprint.

Cite this