Intracytoplasmic sperm injection (ICSI) of prepubertal goat oocytes using fresh and frozen-thawed semen

Irene Menéndez-Blanco, Maria Gracia Catala, Montserrat Roura, Sandra Soto-Heras, Anna Rita Piras, Dolors Izquierdo, Maria Teresa Paramio (Corresponding author)

Research output: Contribution to journalArticleResearchpeer-review

2 Citations (Scopus)

Abstract

The aim of this study was to test the effect of fresh and frozen-thawed semen on embryo development of prepubertal goat oocytes fertilized by intracytoplasmic sperm injection (ICSI). Oocytes were in vitro matured in BO-IVM medium for 26 h. Fresh and frozen-thawed semen were selected by centrifugation in BoviPure(center dot) commercial gradient. Before injection, spermatozoa were assessed for: capacitation through the detection of the tyrosine phosphorylation, viability through propidium iodide stain, and acrosomal status through fluorescein isothiocyanate-conjugated peanut agglutinin stain. Metaphase 11 oocytes were injected with a spermatozoon selected by hyaluronic-acid binding medium (SpermSlow (TM)). Results of frozen-thawed spermatozoa showed significantly higher level of capacitation than fresh spermatozoa (64.92 vs 34.67%, respectively). Moreover fresh semen group showed significantly higher (p <0.02) number of live acrosome reacted sperm (8.84%) than frozen-thawed group (6.42%). At 17 h post ICSI, zygotes (2 pronuclei) were evaluated but no differences were found using fresh and frozen-thawed semen (39.06 vs 37.50% respectively). Moreover, no differences were obtained comparing the percentage of blastocysts produced at day 9 post injection (18.46 vs 12.12%, respectively). In conclusion, either fresh or frozen-thawed semen did not have an effect on the embryo production of prepubertal goat oocytes fertilized by ICSI, despite the differences on viability, acrosomal status and sperm capacitation.

Original languageEnglish
Pages (from-to)137-142
Number of pages6
JournalSmall Ruminant Research
Volume170
DOIs
Publication statusPublished - 1 Jan 2019

Keywords

  • Embryos
  • Ruminants
  • Sperm analysis
  • VIVO
  • PHOSPHORYLATION
  • RISE
  • EMBRYO DEVELOPMENT
  • IN-VITRO
  • HYALURONIC-ACID-BINDING
  • NUCLEI
  • BOAR SPERMATOZOA
  • SELECTION
  • ACROSOME REACTION

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