@article{0d6e805c000a4a7f92b82bdb94ae7a5e,
title = "Intracellular targeting of CD44+ cells with self-assembling, protein only nanoparticles",
abstract = "CD44 is a multifunctional cell surface protein involved in proliferation and differentiation, angiogenesis and signaling. The expression of CD44 is up-regulated in several types of human tumors and particularly in cancer stem cells, representing an appealing target for drug delivery in the treatment of cancer. We have explored here several protein ligands of CD44 for the construction of self-assembling modular proteins designed to bind and internalize target cells. Among five tested ligands, two of them (A5G27 and FNI/II/V) drive the formation of protein-only, ring-shaped nanoparticles of about 14 nm that efficiently bind and penetrate CD44+ cells by an endosomal route. The potential of these newly designed nanoparticles is evaluated regarding the need of biocompatible nanostructured materials for drug delivery in CD44-linked conditions.",
keywords = "Biomaterials, CD44, Drug delivery, Multifunctional protein, Nanoparticle, Self-assembling",
author = "Neus Ferrer-Miralles and Ugutz Unzueta and Petra Gener and Witold Tatkiewicz and Ibane Abasolo and Imma Ratera and Jaume Veciana and Jr, {Sim{\'o} Schwartz} and Antonio Villaverde and Esther Vazquez",
note = "Funding Information: We appreciate the technical support of Fran Cort{\'e}s from the Cell Culture Unit of Servei de Cultius Cel. lulars Producci{\'o} d′Anticossos i Citometria (SCAC, UAB), of the Servei de Microsc{\`o}pia and of Amable Bernab{\'e} from Soft Materials Service (ICMAB-CSIC/CIBER-BBN) and from Proteomics facility from UAB (a member of ProteoRed-ISCIII network). We are also indebted to the Protein Production Platform (CIBER-BBN – UAB) for helpful technical assistance in protein production and purification ( http://www.ciber-bbn.es/en/programas/89-plataforma-de-produccion-de-proteinas-ppp ). The authors also acknowledge the financial support granted to E.V. from FIS ( PI12/00327 ), to S.S. from FIS ( PI11/01079 ), to E.V. and S.S. from The Marat{\'o} de TV3 ( TV32013-133930 ), to J.V. from DGI (Grant POMAs CTQ2010-19501 ) and to A.V. from MINECO ( BIO2013-41019-P ), from Generalitat de Catalunya ( 2014SGR-132 ) and from the Centro de Investigaci{\'o}n Biom{\'e}dica en Red (CIBER) de Bioingenier{\'i}a , Biomateriales y Nanomedicina (NANOPROTHER and PENTRI projects), financed by the Instituto de Salud Carlos III with assistance from the European Regional Development Fund. M.P. and U.U. received Ph.D. fellowships from UAB and from ISCIII respectively. W.T. is grateful to the Consejo Superior de Investigaciones Cient{\'i}ficas (CSIC) for a “JAE-pre” fellowship. A.V. has been distinguished with an ICREA ACADEMIA Award. ",
year = "2014",
month = oct,
day = "1",
doi = "10.1016/j.ijpharm.2014.07.016",
language = "English",
volume = "473",
pages = "286--295",
journal = "International Journal of Pharmaceutics",
issn = "0378-5173",
publisher = "Elsevier",
number = "1-2",
}
