Interleukin-1β stimulates cyclic GMP efflux in brain astrocytes

In rat brain astroglia-enriched cultures long-term treatment with interleukin-1β induces NO release and stimulation of soluble guanylyl cyclase. The cGMP formed is recovered in the extracellular medium but not in the cell monolayer. The interleukin-1β effect is mediated by type I receptor and potentiated by interferon-γ. In cells treated with bacterial endotoxin a larger NO-dependent cGMP accumulation occurs only intracellularly, however a significant cGMP egression is observed when cells are co-treated with interleukin-1β. The non-selective anion transport inhibitors probenecid and verapamil block cGMP efflux, indicating that interleukin-1β stimulates a cGMP transporter. © 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.