Interaction of porcine conventional dendritic cells with swine influenza virus

Tufária Mussá, Carolina Rodriguez-Cariño, Myriam Pujol, Lorena Córdoba, Núria Busquets, Elisa Crisci, Javier Dominguez, Lorenzo Fraile, María Montoya

    Research output: Contribution to journalArticleResearchpeer-review

    14 Citations (Scopus)

    Abstract

    Swine influenza virus (SwIV) causes sub-acute or acute respiratory infections on swine farms and pigs can act as "mixing vessels" for new influenza strains. Knowledge of the immune response of SwIV in its natural host, pigs, is very limited. Dendritic cells (DCs) mediate the induction of immunity to pathogens, but their interaction with SwIV has not been fully characterized. Thus, porcine bone marrow derived DCs (poBMDCs) were exposed to a circulating strain of H3N2 SwIV in vitro. Infection of poBMDCs resulted in structures resembling influenza virus inside poBMDCs in vesicles and also free in cytoplasm. Viral progeny was undetectable in supernatant but limited replication was detected in the first 8. h after infection. However, viral particles from infected-poBMDCs were able to induce cytopathic effect in susceptible cells only when cell-to-cell interaction was favoured. The data generated in our studies reveal the particular interaction of H3N2 SwIV with conventional DCs. © 2011 Elsevier Inc.
    Original languageEnglish
    Pages (from-to)125-134
    JournalVirology
    Volume420
    Issue number2
    DOIs
    Publication statusPublished - 25 Nov 2011

    Keywords

    • Dendritic cells
    • Influenza virus
    • Replication
    • Swine

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