Integrated process for the enzymatic synthesis of the octapeptide PhAcCCK-8

Mercè Fité, Pere Clapés, Josep López-Santín, M. Dolors Benaiges, Glòria Caminal

    Research output: Contribution to journalArticleResearchpeer-review

    13 Citations (Scopus)


    A process for the enzymatic synthesis of PhAcCCK-8 is presented. The CCK-8 (CCK26-33) peptide fragment is the minimum sequence with biological activity of the cholecystokinin hormone. A synthetic convergent strategy has been developed starting from amino acid derivatives as raw materials, employing proteases as biocatalysts for each peptide coupling. The enzymes have been immobilized by deposition onto solid supports in order to be employed in organic media at low water activity. N-Terminal protecting groups such as PhAc, which can be introduced and removed enzymatically, have been employed. The synthesis process has been set up at preparative level with focus in the integration of reaction and separation steps with an overall yield of 15%.
    Original languageEnglish
    Pages (from-to)1214-1220
    JournalBiotechnology Progress
    Publication statusPublished - 1 Nov 2002

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