Influence of water activity and support material on the enzymatic synthesis of a CCK-8 tripeptide fragment

Montserrat Capellas, Ma Dolors Benaiges, Gloria Caminal, Gloria González, Josep López-Santín, Pere Clapés

Research output: Contribution to journalArticleResearchpeer-review

18 Citations (Scopus)

Abstract

The kinetically controlled condensation of Z-Gly-Trp-OMe and H-Met-OEt catalyzed by α-chymotrypsin in organic media is reported. The influence of thermodynamic water activity and the support material used to adsorb α-chymotrypsin, on both the product yield and enzymatic activity was investigated. Polyamide based materials were the best support at low water activity rendering the highest reaction rates and yields. The activity of the adsorbed enzyme at low water activities depends on both the accessible surface area and the hydrophobicity of the support. Polyamide had both adequate hydrophilicity and high surface area yielding the best results. Polypropylene based supports were strongly hydrophobic and, although they presented a high surface area, the enzymatic activity was much lower. The solvents used to carry out the synthesis were acetonitrile and ethyl acetate. No significant differences were observed on the performance of the reaction in either solvent. The tripeptide selected is a fragment of the cholecystokinin C-terminal octapeptide (CCK-8), a biological active peptide involved in the control of gastrointestinal function. © 1996 OPA (Overseas Publishers Association) Amsterdam B.V. Published in The Netherlands by Harwood Academic Publishers GmbH.
Original languageEnglish
Pages (from-to)165-178
JournalBiocatalysis and Biotransformation
Volume13
Issue number3
DOIs
Publication statusPublished - 1 Jan 1996

Keywords

  • Enzymatic peptide synthesis
  • Immobilized α-chymotrypsin
  • Reaction medium engineering
  • Solid support material
  • Solvent-water mixtures
  • Water activity

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