Influence of the substrate's hydrophilicity on the in vitro Schwann cells viability

J. M. Soria; C. Martínez Ramos; O. Bahamonde; D. M. García Cruz; M. Salmerón Sánchez; M. A. García Esparza; C. Casas; M. Guzmán; X. Navarro; J. L. Gómez Ribelles; J. M. García Verdugo; M. Monleón Pradas; J. A. Barcia

doi:https://doi.org/10.1002/jbm.a.31297

Influence of the substrate's hydrophilicity on the in vitro Schwann cells viability

J. M. Soria, C. Martínez Ramos, O. Bahamonde, D. M. García Cruz, M. Salmerón Sánchez, M. A. García Esparza, C. Casas, M. Guzmán, X. Navarro, J. L. Gómez Ribelles, J. M. García Verdugo, M. Monleón Pradas, J. A. Barcia

Department of Cellular Biology, Physiology and Immunology

Research output: Contribution to journal › Article › Research › peer-review

41 Citations (Scopus)

Abstract

A series of polymeric biomaterials including poly(methyl acrylate) (PMA), chitosan (CHT), poly(ethyl acrylate) (PEA), poly(hydroxyethyl acrylate) (PHEA), and a series of random copolymers containing ethyl acrylate and hydroxyethyl acrylate monomeric units were tested in vitro as culture substrates and compared for their impact on the proliferation and expansion of Schwann cells (SCs). Immunocytochemical staining assay and scanning electron microscopy techniques were applied to perform a quantitative analysis to determine the correct maintenance of the cultured glial cells on the different biomaterials. The results strongly suggest that cell attachment and proliferation is influenced by the substrate's surface chemistry, and that hydrophobic biomaterials based on PMA, PEA, and the copolymers PEA and PHEA in a narrow composition window are suitable substrates to promote cell attachment and proliferation of SCs in vitro. © 2007 Wiley Periodicals, Inc.

Original language	English
Pages (from-to)	463-470
Journal	Journal of Biomedical Materials Research - Part A
Volume	83
DOIs	https://doi.org/10.1002/jbm.a.31297
Publication status	Published - 1 Nov 2007

Keywords

Biocompatibility
Copolymer
Hydrogel
Schwann cell
in vitro culture

Access to Document

https://doi.org/10.1002/jbm.a.31297

Cite this

Soria, J. M., Martínez Ramos, C., Bahamonde, O., García Cruz, D. M., Salmerón Sánchez, M., García Esparza, M. A., Casas, C., Guzmán, M., Navarro, X., Gómez Ribelles, J. L., García Verdugo, J. M., Monleón Pradas, M., & Barcia, J. A. (2007). Influence of the substrate's hydrophilicity on the in vitro Schwann cells viability. Journal of Biomedical Materials Research - Part A, 83, 463-470. https://doi.org/10.1002/jbm.a.31297

@article{e1fa4dd1e301449a97abd51213358994,

title = "Influence of the substrate's hydrophilicity on the in vitro Schwann cells viability",

abstract = "A series of polymeric biomaterials including poly(methyl acrylate) (PMA), chitosan (CHT), poly(ethyl acrylate) (PEA), poly(hydroxyethyl acrylate) (PHEA), and a series of random copolymers containing ethyl acrylate and hydroxyethyl acrylate monomeric units were tested in vitro as culture substrates and compared for their impact on the proliferation and expansion of Schwann cells (SCs). Immunocytochemical staining assay and scanning electron microscopy techniques were applied to perform a quantitative analysis to determine the correct maintenance of the cultured glial cells on the different biomaterials. The results strongly suggest that cell attachment and proliferation is influenced by the substrate's surface chemistry, and that hydrophobic biomaterials based on PMA, PEA, and the copolymers PEA and PHEA in a narrow composition window are suitable substrates to promote cell attachment and proliferation of SCs in vitro. {\textcopyright} 2007 Wiley Periodicals, Inc.",

keywords = "Biocompatibility, Copolymer, Hydrogel, Schwann cell, in vitro culture",

author = "Soria, {J. M.} and {Mart{\'i}nez Ramos}, C. and O. Bahamonde and {Garc{\'i}a Cruz}, {D. M.} and {Salmer{\'o}n S{\'a}nchez}, M. and {Garc{\'i}a Esparza}, {M. A.} and C. Casas and M. Guzm{\'a}n and X. Navarro and {G{\'o}mez Ribelles}, {J. L.} and {Garc{\'i}a Verdugo}, {J. M.} and {Monle{\'o}n Pradas}, M. and Barcia, {J. A.}",

year = "2007",

month = nov,

day = "1",

doi = "https://doi.org/10.1002/jbm.a.31297",

language = "English",

volume = "83",

pages = "463--470",

journal = "Journal of Biomedical Materials Research - Part A",

issn = "1549-3296",

publisher = "John Wiley and Sons Inc.",

}

Soria, JM, Martínez Ramos, C, Bahamonde, O, García Cruz, DM, Salmerón Sánchez, M, García Esparza, MA, Casas, C, Guzmán, M, Navarro, X, Gómez Ribelles, JL, García Verdugo, JM, Monleón Pradas, M & Barcia, JA 2007, 'Influence of the substrate's hydrophilicity on the in vitro Schwann cells viability', Journal of Biomedical Materials Research - Part A, vol. 83, pp. 463-470. https://doi.org/10.1002/jbm.a.31297

TY - JOUR

T1 - Influence of the substrate's hydrophilicity on the in vitro Schwann cells viability

AU - Soria, J. M.

AU - Martínez Ramos, C.

AU - Bahamonde, O.

AU - García Cruz, D. M.

AU - Salmerón Sánchez, M.

AU - García Esparza, M. A.

AU - Casas, C.

AU - Guzmán, M.

AU - Navarro, X.

AU - Gómez Ribelles, J. L.

AU - García Verdugo, J. M.

AU - Monleón Pradas, M.

AU - Barcia, J. A.

PY - 2007/11/1

Y1 - 2007/11/1

N2 - A series of polymeric biomaterials including poly(methyl acrylate) (PMA), chitosan (CHT), poly(ethyl acrylate) (PEA), poly(hydroxyethyl acrylate) (PHEA), and a series of random copolymers containing ethyl acrylate and hydroxyethyl acrylate monomeric units were tested in vitro as culture substrates and compared for their impact on the proliferation and expansion of Schwann cells (SCs). Immunocytochemical staining assay and scanning electron microscopy techniques were applied to perform a quantitative analysis to determine the correct maintenance of the cultured glial cells on the different biomaterials. The results strongly suggest that cell attachment and proliferation is influenced by the substrate's surface chemistry, and that hydrophobic biomaterials based on PMA, PEA, and the copolymers PEA and PHEA in a narrow composition window are suitable substrates to promote cell attachment and proliferation of SCs in vitro. © 2007 Wiley Periodicals, Inc.

AB - A series of polymeric biomaterials including poly(methyl acrylate) (PMA), chitosan (CHT), poly(ethyl acrylate) (PEA), poly(hydroxyethyl acrylate) (PHEA), and a series of random copolymers containing ethyl acrylate and hydroxyethyl acrylate monomeric units were tested in vitro as culture substrates and compared for their impact on the proliferation and expansion of Schwann cells (SCs). Immunocytochemical staining assay and scanning electron microscopy techniques were applied to perform a quantitative analysis to determine the correct maintenance of the cultured glial cells on the different biomaterials. The results strongly suggest that cell attachment and proliferation is influenced by the substrate's surface chemistry, and that hydrophobic biomaterials based on PMA, PEA, and the copolymers PEA and PHEA in a narrow composition window are suitable substrates to promote cell attachment and proliferation of SCs in vitro. © 2007 Wiley Periodicals, Inc.

KW - Biocompatibility

KW - Copolymer

KW - Hydrogel

KW - Schwann cell

KW - in vitro culture

U2 - https://doi.org/10.1002/jbm.a.31297

DO - https://doi.org/10.1002/jbm.a.31297

M3 - Article

SN - 1549-3296

VL - 83

SP - 463

EP - 470

JO - Journal of Biomedical Materials Research - Part A

JF - Journal of Biomedical Materials Research - Part A

ER -

Influence of the substrate's hydrophilicity on the in vitro Schwann cells viability

Abstract

Keywords

Access to Document

Fingerprint

Cite this