Influence of the substrate's hydrophilicity on the in vitro Schwann cells viability

J. M. Soria, C. Martínez Ramos, O. Bahamonde, D. M. García Cruz, M. Salmerón Sánchez, M. A. García Esparza, C. Casas, M. Guzmán, X. Navarro, J. L. Gómez Ribelles, J. M. García Verdugo, M. Monleón Pradas, J. A. Barcia

Research output: Contribution to journalArticleResearchpeer-review

36 Citations (Scopus)

Abstract

A series of polymeric biomaterials including poly(methyl acrylate) (PMA), chitosan (CHT), poly(ethyl acrylate) (PEA), poly(hydroxyethyl acrylate) (PHEA), and a series of random copolymers containing ethyl acrylate and hydroxyethyl acrylate monomeric units were tested in vitro as culture substrates and compared for their impact on the proliferation and expansion of Schwann cells (SCs). Immunocytochemical staining assay and scanning electron microscopy techniques were applied to perform a quantitative analysis to determine the correct maintenance of the cultured glial cells on the different biomaterials. The results strongly suggest that cell attachment and proliferation is influenced by the substrate's surface chemistry, and that hydrophobic biomaterials based on PMA, PEA, and the copolymers PEA and PHEA in a narrow composition window are suitable substrates to promote cell attachment and proliferation of SCs in vitro. © 2007 Wiley Periodicals, Inc.
Original languageEnglish
Pages (from-to)463-470
JournalJournal of Biomedical Materials Research - Part A
Volume83
DOIs
Publication statusPublished - 1 Nov 2007

Keywords

  • Biocompatibility
  • Copolymer
  • Hydrogel
  • Schwann cell
  • in vitro culture

Fingerprint Dive into the research topics of 'Influence of the substrate's hydrophilicity on the in vitro Schwann cells viability'. Together they form a unique fingerprint.

  • Cite this

    Soria, J. M., Martínez Ramos, C., Bahamonde, O., García Cruz, D. M., Salmerón Sánchez, M., García Esparza, M. A., Casas, C., Guzmán, M., Navarro, X., Gómez Ribelles, J. L., García Verdugo, J. M., Monleón Pradas, M., & Barcia, J. A. (2007). Influence of the substrate's hydrophilicity on the in vitro Schwann cells viability. Journal of Biomedical Materials Research - Part A, 83, 463-470. https://doi.org/10.1002/jbm.a.31297