Influence of S9 mix in the induction of SOS system by quercetin

Montserrat Llagostera; Salvador Garrido; Jordi Barbé; Ricardo Guerrero; Jose Rueff

doi:10.1016/0165-7992(87)90160-6

Influence of S9 mix in the induction of SOS system by quercetin

Montserrat Llagostera, Salvador Garrido, Jordi Barbé, Ricardo Guerrero, Jose Rueff

Department of Genetics and Microbiology

Research output: Contribution to journal › Article › Research › peer-review

12 Citations (Scopus)

Abstract

The induction of recA, umuC and sfiA genes by quercetin was studied in the presence and in the absence of S9 mix. The inducing activity of quercetin is higher for sfiA than for recA and umuC genes in the absence of S9 mix. The putative genotoxic metabolites of quercetin produced by S9 mix display different inducing activities of the three SOS genes as compared to quercetin. The induction of sfiA gene is decreased by the presence of S9 mix, whereas an opposite effect was observed concerning umuC and recA. These data suggest that the error-prone repair pathway participates in mutagenesis by quercetin and its metabolites. Moreover, the type of DNA damage exerted by quercetin seems to be determined by its metabolic fate. The importance of testing for the induction of other SOS genes, together with sfiA, in the study of SOS functions as a genotoxic index is emphasized. © 1987.

Original language	English
Pages (from-to)	1-4
Journal	Mutation Research Letters
Volume	191
DOIs	https://doi.org/10.1016/0165-7992(87)90160-6
Publication status	Published - 1 Jan 1987

Keywords

Quercetin
RecA gene
S9 mix influence
SOS system induction
SfiA gene
UmuC gene

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10.1016/0165-7992(87)90160-6

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title = "Influence of S9 mix in the induction of SOS system by quercetin",

abstract = "The induction of recA, umuC and sfiA genes by quercetin was studied in the presence and in the absence of S9 mix. The inducing activity of quercetin is higher for sfiA than for recA and umuC genes in the absence of S9 mix. The putative genotoxic metabolites of quercetin produced by S9 mix display different inducing activities of the three SOS genes as compared to quercetin. The induction of sfiA gene is decreased by the presence of S9 mix, whereas an opposite effect was observed concerning umuC and recA. These data suggest that the error-prone repair pathway participates in mutagenesis by quercetin and its metabolites. Moreover, the type of DNA damage exerted by quercetin seems to be determined by its metabolic fate. The importance of testing for the induction of other SOS genes, together with sfiA, in the study of SOS functions as a genotoxic index is emphasized. {\textcopyright} 1987.",

keywords = "Quercetin, RecA gene, S9 mix influence, SOS system induction, SfiA gene, UmuC gene",

author = "Montserrat Llagostera and Salvador Garrido and Jordi Barb{\'e} and Ricardo Guerrero and Jose Rueff",

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doi = "10.1016/0165-7992(87)90160-6",

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T1 - Influence of S9 mix in the induction of SOS system by quercetin

AU - Llagostera, Montserrat

AU - Garrido, Salvador

AU - Barbé, Jordi

AU - Guerrero, Ricardo

AU - Rueff, Jose

PY - 1987/1/1

Y1 - 1987/1/1

N2 - The induction of recA, umuC and sfiA genes by quercetin was studied in the presence and in the absence of S9 mix. The inducing activity of quercetin is higher for sfiA than for recA and umuC genes in the absence of S9 mix. The putative genotoxic metabolites of quercetin produced by S9 mix display different inducing activities of the three SOS genes as compared to quercetin. The induction of sfiA gene is decreased by the presence of S9 mix, whereas an opposite effect was observed concerning umuC and recA. These data suggest that the error-prone repair pathway participates in mutagenesis by quercetin and its metabolites. Moreover, the type of DNA damage exerted by quercetin seems to be determined by its metabolic fate. The importance of testing for the induction of other SOS genes, together with sfiA, in the study of SOS functions as a genotoxic index is emphasized. © 1987.

AB - The induction of recA, umuC and sfiA genes by quercetin was studied in the presence and in the absence of S9 mix. The inducing activity of quercetin is higher for sfiA than for recA and umuC genes in the absence of S9 mix. The putative genotoxic metabolites of quercetin produced by S9 mix display different inducing activities of the three SOS genes as compared to quercetin. The induction of sfiA gene is decreased by the presence of S9 mix, whereas an opposite effect was observed concerning umuC and recA. These data suggest that the error-prone repair pathway participates in mutagenesis by quercetin and its metabolites. Moreover, the type of DNA damage exerted by quercetin seems to be determined by its metabolic fate. The importance of testing for the induction of other SOS genes, together with sfiA, in the study of SOS functions as a genotoxic index is emphasized. © 1987.

KW - Quercetin

KW - RecA gene

KW - S9 mix influence

KW - SOS system induction

KW - SfiA gene

KW - UmuC gene

U2 - 10.1016/0165-7992(87)90160-6

DO - 10.1016/0165-7992(87)90160-6

M3 - Article

SN - 0165-7992

VL - 191

SP - 1

EP - 4

JO - Mutation Research Letters

JF - Mutation Research Letters

ER -

Influence of S9 mix in the induction of SOS system by quercetin

Abstract

Keywords

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