The effect of pH (2 to 10) and temperature (15 and 30°C) on growth and production of ochratoxin A (OTA) of six strains of Aspergillus carbonarius was studied in two culture media: Czapek yeast autolysate agar and yeast extract sucrose agar. Isolates were selected by their different source and different reported ability to produce OTA. Regardless of the initial pH or the temperature tested, Czapek yeast autolysate agar has been shown to be the best culture medium for OTA production by A. carbonarius. In this medium, OTA was produced from pH 2 to 10 at the two incubation temperatures tested. The results obtained show the ability of A. carbonarius to not only grow but also produce OTA over a wide pH range at high or low temperatures. This may help explain why this species is considered the main OTA source in some substrata. Copyright ©, International Association for Food Protection.