Influence of DNA-repair gene variants on the micronucleus frequency in thyroid cancer patients

W. A. García-Quispes, S. Pastor, P. Galofré, F. Biarnés, J. Castell, A. Velázquez, R. Marcos

Research output: Contribution to journalArticleResearchpeer-review

8 Citations (Scopus)

Abstract

The role of different DNA-repair genes (OGG1, XRCC1, XRCC2 and XRCC3) on both the spontaneous and the induced frequency of micronuclei (MN) has been studied in the lymphocytes of a group of 114 patients with differentiated thyroid cancer (DTC). Induction of MN was achieved by treatment of the lymphocytes with 0.5. Gy of gamma-radiation.The selected genes are involved in base-excision repair (BER) (OGG1, Ser326Cys; XRCC1, Arg280His and Arg399Gln), and in homologous recombination repair (HRR) (XRCC2, Arg188His and XRCC3, IVS5-14G). Genotyping was carried out by use of the iPLEX (Sequenom) technique.Results indicate that only the OGG1-Ser326Cys polymorphism was able to modulate the MN frequency. This effect was only observed in the spontaneous MN frequency (P=0.016), but not in the MN frequency induced after irradiation. In addition, a strong correlation was observed between spontaneous and induced MN frequency, which would suggest an underlying genetic background. © 2012 Elsevier B.V.
Original languageEnglish
Pages (from-to)34-39
JournalMutation Research - Genetic Toxicology and Environmental Mutagenesis
Volume750
DOIs
Publication statusPublished - 20 Jan 2013

Keywords

  • Genetic polymorphisms
  • Human lymphocytes
  • Micronucleus
  • Thyroid cancer

Fingerprint Dive into the research topics of 'Influence of DNA-repair gene variants on the micronucleus frequency in thyroid cancer patients'. Together they form a unique fingerprint.

Cite this