TY - JOUR
T1 - Inflammation affects the viability and plasticity of equine mesenchymal stem cells: Possible implications in intra-articular treatments
AU - Barrachina, Laura
AU - Remacha, Ana Rosa
AU - Romero, Antonio
AU - Vázquez, Francisco José
AU - Albareda, Jorge
AU - Prades, Marta
AU - Ranera, Beatriz
AU - Zaragoza, Pilar
AU - Martín-Burriel, Inmaculada
AU - Rodellar, Clementina
PY - 2017/3/1
Y1 - 2017/3/1
N2 - © 2017 The Korean Society of Veterinary Science. All Rights Reserved. Mesenchymal stem cells (MSCs) are gaining relevance for treating equine joint injuries because of their ability to limit inflammation and stimulate regeneration. Because inflammation activates MSC immunoregulatory function, proinflammatory priming could improve MSC efficacy. However, inflammatory molecules present in synovial fluid or added to the culture medium might have deleterious effects on MSCs. Therefore, this study was conducted to investigate the effects of inflammatory synovial fluid and proinflammatory cytokines priming on viability and plasticity of equine MSCs. Equine bone marrow derived MSCs (eBM-MSCs) from three animals were cultured for 72 h in media supplemented with: 20% inflammatory synovial fluid (SF); 50 ng/mL IFN-γ and TNF-α (CK50); and 20 ng/mL IFN-γ and TNF-α (CK20). Proliferation assay and expression of proliferation and apoptosis-related genes showed that SF exposed-eBM-MSCs maintained their viability, whereas the viability of CK primed-eBM-MSCs was significantly impaired. Tri-lineage differentiation assay revealed that exposure to inflammatory synovial fluid did not alter eBM-MSCs differentiation potential; however, eBM-MSCs primed with cytokines did not display osteogenic, adipogenic or chondrogenic phenotype. The inflammatory synovial environment is well tolerated by eBM-MSCs, whereas cytokine priming negatively affects the viability and differentiation abilities of eBM-MSCs, which might limit their in vivo efficacy.
AB - © 2017 The Korean Society of Veterinary Science. All Rights Reserved. Mesenchymal stem cells (MSCs) are gaining relevance for treating equine joint injuries because of their ability to limit inflammation and stimulate regeneration. Because inflammation activates MSC immunoregulatory function, proinflammatory priming could improve MSC efficacy. However, inflammatory molecules present in synovial fluid or added to the culture medium might have deleterious effects on MSCs. Therefore, this study was conducted to investigate the effects of inflammatory synovial fluid and proinflammatory cytokines priming on viability and plasticity of equine MSCs. Equine bone marrow derived MSCs (eBM-MSCs) from three animals were cultured for 72 h in media supplemented with: 20% inflammatory synovial fluid (SF); 50 ng/mL IFN-γ and TNF-α (CK50); and 20 ng/mL IFN-γ and TNF-α (CK20). Proliferation assay and expression of proliferation and apoptosis-related genes showed that SF exposed-eBM-MSCs maintained their viability, whereas the viability of CK primed-eBM-MSCs was significantly impaired. Tri-lineage differentiation assay revealed that exposure to inflammatory synovial fluid did not alter eBM-MSCs differentiation potential; however, eBM-MSCs primed with cytokines did not display osteogenic, adipogenic or chondrogenic phenotype. The inflammatory synovial environment is well tolerated by eBM-MSCs, whereas cytokine priming negatively affects the viability and differentiation abilities of eBM-MSCs, which might limit their in vivo efficacy.
KW - Horses
KW - Joint diseases
KW - Mesenchymal stromal cells
KW - Proinflammatory cytokines
KW - Synovial fluid
UR - https://ddd.uab.cat/record/185897
U2 - https://doi.org/10.4142/jvs.2017.18.1.39
DO - https://doi.org/10.4142/jvs.2017.18.1.39
M3 - Article
VL - 18
SP - 39
EP - 49
JO - Journal of Veterinary Science
JF - Journal of Veterinary Science
SN - 1229-845X
IS - 1
ER -