Induction of the SOS response by hydroxyurea in Escherichia coli K12

Jordi Barbé; Antonio Villaverde; Ricardo Guerrero

doi:https://doi.org/10.1016/0165-7992(87)90105-9

Induction of the SOS response by hydroxyurea in Escherichia coli K12

Jordi Barbé, Antonio Villaverde, Ricardo Guerrero

Research output: Contribution to journal › Article › Research › peer-review

19 Citations (Scopus)

Abstract

Hydroxyurea at concentrations higher than 10-2 M induced the recA and sfiA genes of E. coli as well as the lambda prophage by a pathway independent of the recBC genes. In addition, the hydroxyurea-mediated induction of the SOS response is accompanied by a recA-dependent decrease on the cellular ATP pool. The presence of the multicopy plasmid pPS2, harboring the nrdAB genes (encoding the ribonucleoside reductase enzyme), abolished the hydroxyurea-induced expression of the recA gene. These data lead us to suggest that induction of the SOS response by hydroxyurea is due to the blocking of DNA replication by the inhibition of the ribonucleoside reductase complex activity. © 1987.

Original language	English
Pages (from-to)	105-108
Journal	Mutation Research Letters
Volume	192
DOIs	https://doi.org/10.1016/0165-7992(87)90105-9
Publication status	Published - 1 Jan 1987

Keywords

(Escherichia coli)
Hydroxyurea
Lambda prophage
SOS response, induction

Access to Document

https://doi.org/10.1016/0165-7992(87)90105-9

Cite this

@article{050746dcf574404a866ad44e4ab04040,

title = "Induction of the SOS response by hydroxyurea in Escherichia coli K12",

abstract = "Hydroxyurea at concentrations higher than 10-2 M induced the recA and sfiA genes of E. coli as well as the lambda prophage by a pathway independent of the recBC genes. In addition, the hydroxyurea-mediated induction of the SOS response is accompanied by a recA-dependent decrease on the cellular ATP pool. The presence of the multicopy plasmid pPS2, harboring the nrdAB genes (encoding the ribonucleoside reductase enzyme), abolished the hydroxyurea-induced expression of the recA gene. These data lead us to suggest that induction of the SOS response by hydroxyurea is due to the blocking of DNA replication by the inhibition of the ribonucleoside reductase complex activity. {\textcopyright} 1987.",

keywords = "(Escherichia coli), Hydroxyurea, Lambda prophage, SOS response, induction",

author = "Jordi Barb{\'e} and Antonio Villaverde and Ricardo Guerrero",

year = "1987",

month = jan,

day = "1",

doi = "https://doi.org/10.1016/0165-7992(87)90105-9",

language = "English",

volume = "192",

pages = "105--108",

journal = "Mutation Research Letters",

issn = "0165-7992",

publisher = "Elsevier BV",

}

TY - JOUR

T1 - Induction of the SOS response by hydroxyurea in Escherichia coli K12

AU - Barbé, Jordi

AU - Villaverde, Antonio

AU - Guerrero, Ricardo

PY - 1987/1/1

Y1 - 1987/1/1

N2 - Hydroxyurea at concentrations higher than 10-2 M induced the recA and sfiA genes of E. coli as well as the lambda prophage by a pathway independent of the recBC genes. In addition, the hydroxyurea-mediated induction of the SOS response is accompanied by a recA-dependent decrease on the cellular ATP pool. The presence of the multicopy plasmid pPS2, harboring the nrdAB genes (encoding the ribonucleoside reductase enzyme), abolished the hydroxyurea-induced expression of the recA gene. These data lead us to suggest that induction of the SOS response by hydroxyurea is due to the blocking of DNA replication by the inhibition of the ribonucleoside reductase complex activity. © 1987.

AB - Hydroxyurea at concentrations higher than 10-2 M induced the recA and sfiA genes of E. coli as well as the lambda prophage by a pathway independent of the recBC genes. In addition, the hydroxyurea-mediated induction of the SOS response is accompanied by a recA-dependent decrease on the cellular ATP pool. The presence of the multicopy plasmid pPS2, harboring the nrdAB genes (encoding the ribonucleoside reductase enzyme), abolished the hydroxyurea-induced expression of the recA gene. These data lead us to suggest that induction of the SOS response by hydroxyurea is due to the blocking of DNA replication by the inhibition of the ribonucleoside reductase complex activity. © 1987.

KW - (Escherichia coli)

KW - Hydroxyurea

KW - Lambda prophage

KW - SOS response, induction

U2 - https://doi.org/10.1016/0165-7992(87)90105-9

DO - https://doi.org/10.1016/0165-7992(87)90105-9

M3 - Article

VL - 192

SP - 105

EP - 108

JO - Mutation Research Letters

JF - Mutation Research Letters

SN - 0165-7992

ER -

Induction of the SOS response by hydroxyurea in Escherichia coli K12

Abstract

Keywords

Access to Document

Fingerprint

Cite this