Induction of the blue form of bacteriohodopsin by low concentrations of sodium dodecyl sulfate

Esteve Padrós, Mireia Duñach, Manuel Sabés

Research output: Contribution to journalArticleResearchpeer-review

28 Citations (Scopus)

Abstract

The effects produced on bacteriorhodopsin by low concentrations of several detergents have been studied by absorption and fourth-derivative spectrophotometry. Sodium dodecyl sulfate induces the appearance of the blue form of bacteriorhodopsin (λmax = 600 nm) at pH values up to 7.0 in a reversible manner. The apparent pK of the purple-to-blue transition raised with increasing concentration of SDS. Of the other detergents tested, only sodium dodecyl-N-sarcosinate showed a slight red-shift of the absorption band to 580 nm, whereas sodium taurocholate, Triton X-100 and cetyltrimethylammonium bromide did not favour the appearance of the blue form. The effect of SDS was found to be consistent with a localized conformational change that moves away the counter-ion of the protonated Schiff base. © 1984.
Original languageEnglish
Pages (from-to)1-7
JournalBBA - Biomembranes
Volume769
DOIs
Publication statusPublished - 11 Jan 1984

Keywords

  • (H. halobium)
  • Bacteriorhodopsin
  • Detergent-induced transition
  • Fourth-derivative spectrophotometry

Fingerprint

Dive into the research topics of 'Induction of the blue form of bacteriohodopsin by low concentrations of sodium dodecyl sulfate'. Together they form a unique fingerprint.

Cite this