Abstract
The effects produced on bacteriorhodopsin by low concentrations of several detergents have been studied by absorption and fourth-derivative spectrophotometry. Sodium dodecyl sulfate induces the appearance of the blue form of bacteriorhodopsin (λmax = 600 nm) at pH values up to 7.0 in a reversible manner. The apparent pK of the purple-to-blue transition raised with increasing concentration of SDS. Of the other detergents tested, only sodium dodecyl-N-sarcosinate showed a slight red-shift of the absorption band to 580 nm, whereas sodium taurocholate, Triton X-100 and cetyltrimethylammonium bromide did not favour the appearance of the blue form. The effect of SDS was found to be consistent with a localized conformational change that moves away the counter-ion of the protonated Schiff base. © 1984.
Original language | English |
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Pages (from-to) | 1-7 |
Journal | BBA - Biomembranes |
Volume | 769 |
DOIs | |
Publication status | Published - 11 Jan 1984 |
Keywords
- (H. halobium)
- Bacteriorhodopsin
- Detergent-induced transition
- Fourth-derivative spectrophotometry