Induction of mutations by tritiated water and 3H-thymidine in Drosophila melanogaster assayed by the somatic zeste-white eye mutation system

Åksa Rasmuson, Noel Xamena, Amadeo Creus, Ricardo Marcos

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5 Citations (Scopus)

Abstract

In order to study the mutagenic effect of exposure to tritium, Drosophila melanogaster larvae were treated with tritiated water (3H2O) or tritiated thymidine (3H-TdR) during development. Dose rates ranged from 0.0058 to 0.058 rad/h per nucleus for 3H-TdR and from 0.049 to 0.122 rad/h for 3H2O. Induction of mutations was measured by the appearance of somatic mutations in the eyes of an unstable strain of Drosophila melanogaster. Both substances caused a significant increase in mutation frequency. With the assumption that each mutation observed in this assay is caused by one DNA break, the effectiveness of tritium to create DNA breaks is estimated to be 0.20 breaks per decay for 3H-TdR and 0.27 breaks per decay for 3H2O. © 1988.
Original languageEnglish
Pages (from-to)127-133
JournalMutation Research Letters
Volume207
Issue number3-4
DOIs
Publication statusPublished - 1 Jan 1988

Keywords

  • Drosophila melanogaster
  • Somatic mutations
  • Tritiated thymidine
  • Tritiated water

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