Increased numbers of myeloid and lymphoid IL-10 producing cells in spleen of pigs with naturally occurring postweaning multisystemic wasting syndrome

E. Crisci, M. Ballester, J. Domínguez, J. Segalés, M. Montoya

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9 Citations (Scopus)

Abstract

Porcine circovirus type 2 (PCV2) is the essential etiological agent of postweaning multisystemic wasting syndrome (PMWS), a worldwide distributed pig disease. The involvement of the immune system in the pathogenesis of PMWS is considered crucial. Previous studies have shown a cytokine profile suggesting T immunosuppression and indicating that interleukin 10 (IL-10) may play an important role during PCV2 infection. Nine 11- to 12-week-old conventional pigs were obtained from commercial farms located in North-Eastern Spain with historical records of PMWS. Spleen from four healthy and five PMWS-affected animals were collected at the necropsy. Viral load was determined in serum by means of standard PCR and real-time quantitative PCR. Phenotype and distribution of different immune cells involved in IL-10 secretion in the spleen of studied pigs were analysed using immunofluorescent assays. The CD163+, CD4+, and CD8+ cell subpopulations produced IL-10 in the spleen and IL-10+ cell numbers were higher in PMWS animals compared with their healthy counterparts. Furthermore, IL-10 producing cells were not infected by PCV2 and were mainly localized in the periarteriolar lymphoid sheaths. This is the first immunophenotyping study on IL-10 producing cells in cases of PMWS, further extending the studies on the role of IL-10 in disease pathogenesis. © 2010 Elsevier B.V.
Original languageEnglish
Pages (from-to)305-310
JournalVeterinary Immunology and Immunopathology
Volume136
DOIs
Publication statusPublished - 15 Aug 2010

Keywords

  • Immunofluorescence assay
  • Interleukin-10
  • Porcine circovirus type 2
  • Postweaning multisystemic wasting syndrome
  • Spleen

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