TY - JOUR
T1 - Inactivation study of Bacillus subtilis, Geobacillus stearothermophilus, Alicyclobacillus acidoterrestris and Aspergillus niger spores under Ultra-High Pressure Homogenization, UV-C light and their combination
AU - Reverter-Carrión, L.
AU - Sauceda-Gálvez, J. N.
AU - Codina-Torrella, I.
AU - Hernández-Herrero, M. M.
AU - Gervilla, R.
AU - Roig-Sagués, A. X.
PY - 2018/8/1
Y1 - 2018/8/1
N2 - © 2018 Elsevier Ltd Lethality rates of Bacillus subtilis, Geobacillus stearothermophilus, Alicyclobacillus acidoterrestris, and Aspergillus niger spores were studied into phosphate-buffer saline (PBS) and PBS supplemented with caramel (PBSC) after Ultra-High Pressure Homogenization (UHPH) and UV-C treatments applied separately and combined. UHPH procedures were carried out at different pressures (100, 200 and 300 MPa) and inlet temperatures (20, 50 and 70 °C), while UV-C treatments consisted in a combination of doses (from 0.9 to 21.5 J/mL) and eventually temperatures (20 and 50 °C) or cycles. UHPH treatments were efficient at inactivating A. niger conidiospores in PBS but much less efficient in the inactivation of bacterial spores. Efficacy of UHPH against bacterial spores was increased pre-heating the samples at 70 °C. UV-C was more efficient against bacterial spores in PBS, but much less in PBSC. When UV-C treatments were applied through several cycles their efficacy against B. subtilis spores increased. Combination of UHPH at 200 MPa and UV-C moderately increased the lethal effect on B. subtilis spores than when these treatments were applied separately. Nevertheless, the combination of both technologies has a complementary effect allowing acting on a wide range of microorganisms, increasing the possibilities to obtain a safer and durable product.
AB - © 2018 Elsevier Ltd Lethality rates of Bacillus subtilis, Geobacillus stearothermophilus, Alicyclobacillus acidoterrestris, and Aspergillus niger spores were studied into phosphate-buffer saline (PBS) and PBS supplemented with caramel (PBSC) after Ultra-High Pressure Homogenization (UHPH) and UV-C treatments applied separately and combined. UHPH procedures were carried out at different pressures (100, 200 and 300 MPa) and inlet temperatures (20, 50 and 70 °C), while UV-C treatments consisted in a combination of doses (from 0.9 to 21.5 J/mL) and eventually temperatures (20 and 50 °C) or cycles. UHPH treatments were efficient at inactivating A. niger conidiospores in PBS but much less efficient in the inactivation of bacterial spores. Efficacy of UHPH against bacterial spores was increased pre-heating the samples at 70 °C. UV-C was more efficient against bacterial spores in PBS, but much less in PBSC. When UV-C treatments were applied through several cycles their efficacy against B. subtilis spores increased. Combination of UHPH at 200 MPa and UV-C moderately increased the lethal effect on B. subtilis spores than when these treatments were applied separately. Nevertheless, the combination of both technologies has a complementary effect allowing acting on a wide range of microorganisms, increasing the possibilities to obtain a safer and durable product.
KW - Alicyclobacillus acidoterrestris
KW - Aspergillus niger
KW - Bacillus subtilis
KW - Geobacillus stearothermophilus
KW - UV-C light radiation
KW - Ultra-High Pressure Homogenization (UHPH)
UR - http://www.scopus.com/inward/record.url?scp=85049633296&partnerID=8YFLogxK
U2 - 10.1016/j.ifset.2018.06.011
DO - 10.1016/j.ifset.2018.06.011
M3 - Article
VL - 48
SP - 258
EP - 264
JO - Innovative Food Science and Emerging Technologies
JF - Innovative Food Science and Emerging Technologies
SN - 1466-8564
ER -